【摘要】 采用正交试验设计的方法,对白术ISSR-PCR反应体系进行优化,确立了适合于白术的ISSR-PCR反应的最佳体系,即在25μL反应体系中,10×buffer 2.5μL,Mg2+3 mmol/L,Taq酶0.75U,模板200 ng,dNTP 0.3mmol/L,引物0.5μmol/L,通过梯度PCR试验得到相应引物的最佳退火温度为55.0℃。更多还原

【Abstract】 The orthogonal design was used to optimize ISSR-PCR reaction system of Atractylodes macrocephala Koidz.A suitable ISSR-PCR system was established and a total volume of 25 μL ISSR-PCR system consisted of 10×buffer 2.5 μL,Taq DNA polymerase 0.75U,Mg2+3 mmol/L,template DNA 200 ng,dNTP 0.3 mmol/L,primer 0.5 μmol/L.The optimal annealing temperature of ISSR-PCR for Atractylodes macrocephala Koidz reaction was proposed by gradient PCR.更多还原