【摘要】 目的观察胰高血糖素样肽2对小鼠胃肠道黏膜的作用效果;检测胰高血糖素样肽2受体在小鼠不同脏器的分布和表达。方法选用5～6周龄的雄性健康BALB/C小鼠,体重17～20 g。随机分为对照组(8只)和胰高血糖素样肽处理组(9只)两组。胰高血糖素样肽2处理组小鼠,每天按250μg/kg体重腹腔注射胰高血糖素样肽2液两次,连续3 d;对照组小鼠每天腹腔注射等量生理盐水两次,连续3 d。3 d后,处死小鼠,每组抽取样本用于形态学观察。将组织切片进行HE染色,观察小鼠胃肠黏膜的组织学变化,用免疫组织化学方法检测胰高血糖素样肽2受体在胃肠组织的表达和分布。结果小鼠经胰高血糖素样肽2处理后,胃黏膜上皮增厚,胃小凹加深;小肠不同肠段黏膜的绒毛高度较对照组明显增加(p<0.05);绒毛增高,隐窝加深;结肠黏膜上皮增厚;胰高血糖素样肽2受体在胃肠道各部位均有表达,而肝、肺、肾与膀胱显阴性。结论胰高血糖素样肽2可能刺激胃黏膜与结肠黏膜上皮增厚,增加小肠不同肠段黏膜的绒毛高度;小鼠的胃、十二指肠、空肠、回肠与结肠均有胰高血糖素样肽2受体的表达,肝、肺、肾与膀胱无胰高血糖素样肽2受体的表达。更多还原

【Abstract】 Objective The effect of glucagon like peptide 2(GLP-2) on gastrointestinal mucosa is observed and expression and distribution of GLP-2 receptor(GLP-2R) are examined in the different parts of different organs in mice.Methods Male and healthy BALB/C mice(5-6 weeks old) weighing 17-20 g are used.The mice are divided into control group(n=8) and GLP-2 treated group(n=9) randomly.GLP-2 treated group mice are given GLP-2(250 μg/kg) intraperitoneal injection twice per day for three days.Control group mice are given physiological saline intraperitoneal injection twice per day for three days.Three days later,mice are sacrificedand samples are taken in each group for morphological observations.The histological changes of gastrointestinal mucosa in mice are observed by dyeing with hematoxylin and eosin staining.The expression and distribution of GLP-2R in gastrointestinal tissue are detected by Immunohistochemistry.Results After treating with GLP-2,the muscosa epithelium of stomach thickens and gastric pit deepens in mice;Mucosal villus in different section of small intestine grow obviously higher in GLP-2 treated group compared with control group(p<0.05);Villus in small intestinal segment grows higher,crypt deepens,and colon mucosa epithelium thickens;GLP-2R is widely expressed in each part of gastrointestinal tract in mice.The expression of GLP-2R is not observed in liver,lung,kidney and bladder.Conclusion GLP-2 is able to stimulate gastric mucosa and colon epithelium mucosa to grow and increase mucosal villus height in different section of smallintestine;GLP-2R is widely expressed gastric,duodenal,jejunum,ileum and colon in mice,the expression of GL P-2R in liver,lung,kidney and bladder was not observed.更多还原