【摘要】 [目的]探讨Rh2抗PC-3(前列腺癌细胞株)以及Rh2与DDP抗PC-3细胞有无协同作用。[方法]采用MTT比色法观察药物对癌细胞的杀伤作用;免疫细胞化学技术观察Bcl-2在各组之间表达情况;流式细胞术分析细胞周期。[结果]不同浓度Rh2与PC-3共同孵育24h后,Rh2对PC-3生长抑制率随剂量增加而升高,呈剂量依赖性。免疫细胞学染色结果显示,随Rh2剂量增加细胞中的Bcl-2基因表达下调。流式细胞术结果显示Rh2可以使PC-3细胞周期阻滞在G1期。[结论]Rh2和DDP显示了明显的协同作用,为前列腺癌化疗药的选择与组方提供了重要的参考。更多还原

【Abstract】 [Objective] To study the effect of Rh2 on PC-3 in vivo and in vitro,and the feasibility of synergism in it induced by Rh2 and DDP.[Methods] The effect of killing tumor cell of drug by MTT were measured;The Bcl-2 difference among groups were determined with immunohistochemistry;The changing of cell cycle were analyzed by using flow cytometry(FCM).[Results] After PC-3 cells were treated with Rh2 in different concentration,Rh2 could significantly inhibit PC-3 growth in vitro.The inhibitory rate increased with the increasing of Rh2 in a dose-dependent.Rh2 could down-regulate the expression of Bcl-2 in PC-3 cells.Rh2 could stop PC-3 in G1 phase during cell cycle.[Conclusion] Rh2 and DDP had obviously synergism function.Our study would provide the scientific data in choosing chemotherapeutic drugs in treatment of prostatic cancer.更多还原