【摘要】 [目的]探讨PARP-1抑制剂PJ34干预治疗对大鼠局灶脑缺血/再灌注损伤的影响。[方法]用线栓法建立局灶脑缺血/再灌注模型(Ⅰ组),分别经左侧侧脑室注射PJ34(Ⅱ组)、生理盐水(Ⅲ组)和假手术组(Ⅳ组);通过Klenow标记及TUNEL染色技术检测鼠脑中单、双链DNA断裂,分别用原位杂交及免疫组化技术检测鼠脑中PARP-1蛋白的表达,同时测定脑组织及血浆中TNF–α、IL-6及IL-8含量变化和细胞凋亡情况。[结果]与Ⅰ组比较,Ⅲ组鼠脑缺血侧DNA单、双链断裂,PARP-1的表达均无明显差异,Ⅱ组缺血侧脑中Klenow及TUNEL阳性细胞数均明显减少,PARP-1的表达明显减少,TNF-α、IL-6及IL-8含量和凋亡减少。[结论]PARP-1抑制剂PJ34对脑缺血/再灌注损伤具有保护作用。更多还原

【Abstract】 [Objective] To investigate the effect of PARP-1inhibitor PJ34 on the focal cerebral ischemia-reperfusion injury. [Methods] Thread embolism method was used to develop the model of focal cerebral ischemia-reperfusion injury in rats (Ⅰgroup), then PJ34 or normal sodium was injected through left lateral ventricles (Ⅱ group and Ⅲ group) and establish sham operation group (Ⅳ group); Klenow marking and TUNEL staining technique were taken to estimate DNA single and double strands fragmentation, and PARP-1 expressions was determined by situ hybridization and immunohistochemistry technique. The changes of TNF-α, IL-6 and IL-8 in brain tissues and in plasma were dynamically observed. [Results] There was no significant difference in DNA single and double strands fragmentation and PARP-1 expressions between the model group and solvent group. PJ34 could decrease significantly the positive cell of Klenow and TUNEL, expression of TNF-α, IL-6, IL-8 and the neuronal apoptosis in cerebral ischemic side. [Conclusion] PARP-1 might have protective effect on cerebral ischemia- reperfusion injury.更多还原