【摘要】 目的建立灯盏花素缓释胶囊中野黄芩苷的高效液相色谱法(HPLC)含量测定方法。方法采用高效液相色谱法,C18柱色谱柱(4.6mm×150mm,5μm),柱温30℃;进样量10μL,流动相:甲醇-四氢呋喃-0.1%磷酸溶液(14:14:72),流速1.0mL·min-1;检测波长335nm。结果野黄芩苷在24.45～293.46μg·mL-1范围内,峰面积与浓度的线性关系良好(r=0.9999),野黄芩苷平均回收率为101.5%,RSD为3.616%。结论该方法简便准确,重现性好,可用于灯盏花素缓释胶囊的质量控制。更多还原

【Abstract】 OBJECTIVE To develop an HPLC method of scutellarin in brevisapin sustained-release capsules.METHODS C18(4.6 mm ×150 mm,5 μm) column with temperature of 30 ℃ was used.The mobile phase was the mixed solution of methanol-tetrahydrofuran-0.1% phosphoric acid(14:14:72) with the flow rate of 1.0 mL·min-1.Determination wavelength was 335 nm and the sample injected volume was 10 μL.RESULTS A good linearity of scutellarin was in the range of 24.45-293.46 μg·mL-1.The average recovery of scutellarin was 101.5% with RSD=3.616%.CONCLUSION The method is simple,accurate,and can be used for the quality control of brevisapin sustained-release capsules.更多还原