【摘要】 目的建立用HPLC同时测定复方益肝灵片中水飞蓟宾、五味子醇甲的含量。方法Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水,梯度洗脱为0→60 min(甲醇40%→75%,水60%→25%),检测波长:水飞蓟宾287 nm,五味子醇甲250 nm。结果水飞蓟宾和五味子醇甲分别在4.96~39.68μg.mL-1(r=0.999 8)和4.00~32.00μg.mL-1(r=0.999 8)内呈良好的线性关系,平均加样回收率分别为98.8%和99.5%,RSD分别为0.96%和0.59%。结论该方法简便、快速、重复性好,可用于复方益肝灵片的质量控制。更多还原

【Abstract】 OBJECTIVE To simultaneous determine the content of silymarin and schizandrol in compound Yi Ganling tablets by HPLC.METHODS The analytical column was a Diamonsil C18 column(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-water.The gradient condition was 0→60 min,methanol 40%→75% and water 60%→25%.The detection wavelengths of silymarin and schizandrol were at 287 nm and 250 nm.RESULTS The calibration curves of silymarin and schizandrol were in good linearity over the ranges of 4.96-39.68 μg·mL-1(r=0.999 8) and 4.00-32.00 μg·mL-1(r=0.999 8),the average recoveries were 98.8% and 99.5%,RSD were 0.96% and 0.59%.CONCLUSION The method is simple,rapid,reliable and can be used for the drug control.更多还原