【摘要】 目的建立克泻灵片中生物碱成分的大鼠血浆HPLC同时测定方法。方法灌胃克泻灵片,以乙酰苯胺为内标,RP-HPLC-UV法在220nm波长处进行血药浓度测定。结果槐定碱和苦参碱在大鼠血浆中定量准确,最低检测限分别为350ng.mL-1和100ng.mL-1,回收率大于98%。结论该方法简便、准确、重复性好,可为克泻灵片的体内药动学研究提供方法学参考。更多还原

【Abstract】 OBJECTIVE To establish an HPLC method for simultaneously determining the alkaloids of Kexieling tablets in rat plasma.METHODS The plasma samples were determined at 220 nm wavelength by reversed-phase HPLC and ultraviolet detection following oral administration of Kexieling tablets.RESULTS Sophoridine and matrine with 350 and 100 ng·mL-1 of limits of quantitation in plasma,respectively,can be accurately quantitated,and the recovery of both analytes was greater than 98%.CONCLUSION The method is simple,accurate and can be referenced for pharmacokinetic study of Kexieling tablets.更多还原