【摘要】 目的研究COX-2及PINCH蛋白在口腔鳞状细胞癌中的表达情况,探讨二者在口腔鳞状细胞癌中表达的相关性。方法采用免疫组织化学ABC法,检测20例正常口腔粘膜和57例口腔鳞状细胞癌石蜡存档标本中COX-2及PINCH蛋白的表达情况。结果COX-2蛋白在正常口腔粘膜中少量表达,而在口腔鳞状细胞癌中的表达明显增加,二者比较有显著性差异;在口腔鳞状细胞癌中,淋巴结转移组阳性表达率为73%(19/26),淋巴结无转移组阳性表达率为29%(9/31),二者差异有显著性;COX-2蛋白的表达与患者性别、年龄、肿瘤大小、部位和分化程度无相关性;COX-2与PINCH有明显的相关性。结论COX-2蛋白在口腔鳞状细胞癌的发生、发展中可能起着重要作用,并与口腔鳞状细胞癌的淋巴结转移相关,其转移机制可能与通过PINCH蛋白促进淋巴管生成有关。更多还原

【Abstract】 Objective To study the COX-2 and PINCH Protein expression in oral squamous cell carcinoma and the relationship between them.Methods ABC immunohistochemical method was used to investigate the expression of COX-2 and PINCH protein in paraffin block of 20 normal oral mucous tissue and 57 oral squamous cell carcinoma(SCC) cases.Analyze the relationship between the expression of COX-2 and the patients clinical variables and its relationship with PINCH protein.Results Immunostaining of COX-2 was low in normal oral mucous tissue and increased in oral SCC.The statistically difference was significant(P<0.05).The positive rate of COX-2 expression was 73%(12/26) and 29%(9/31) of the lymph node metastasis group and the non-metastasis group respectively(P<0.05).The COX-2 expression was not obviously correlated with patients gender,differentiation,age,location and tumor size(P>0.05).COX-2 has a positive relationship with PINCH protein.Conclusion COX-2 may play an important role in the tumorgenesis and development of oral SCC,COX-2 may be related with the lymph node metastasis of OSCC and related with PINCH protein which can promote the lymphangenesis.更多还原