【摘要】 背景:建立有效的完全性脊髓损伤动物模型是深入研究脊髓损伤的前提,只有建立标准的、可重复性高的实验动物模型才能择优选出治疗脊髓损伤的可行方案。目的:实验拟建立一种稳定的大鼠完全性脊髓损伤动物模型。设计、时间及地点:对照观察动物实验,于2007-11/2008-10在石河子大学药学院动物试验中心完成。材料:30只健康Wistar大鼠随机分成假手术组6只、实验组24只。方法:显露实验组大鼠T8～T12棘突及椎板,切除T9～10棘突及椎板,暴露相应脊髓段作为损伤区,采用大鼠脑定位仪自主设计改良Allen模型打击装置,予15g×20cm=2.94×10-2N重力打击大鼠T10节段脊髓,动物模型保证硬脊膜完整。假手术组仅同法暴露相应脊髓段,但不做打击。主要观察指标:造模后2,4,8周以斜板试验及BBB评分观察大鼠双后肢运动功能,以苏木精-伊红染色观察大鼠脊髓组织的变化。结果:假手术组大鼠苏醒后能站立行走,斜板试验角度均大于70°,BBB评分21分,脊髓结构正常。实验组大鼠造模后双下肢全瘫,2只大鼠表现为痉挛性瘫痪,5只大鼠表现出不同程度的自残现象。造模后2,4,8周斜板试验角度均小于30°,BBB评分均少于10分,随时间延长,部分大鼠可见后肢刺激性反射,但无主动性功能活动,局部脊髓结构破坏严重。结论:以2.94×10-2N重力打击大鼠脊髓可保证硬脊膜的完整,并获得稳定的完全性脊髓损伤动物模型。更多还原

【Abstract】 BACKGROUND: Establishing an effective and complete animal model is a precondition for further research of spinal cord injury (SCI), which can provide a basis for SCI treatment. OBJECTIVE: To establish a stable animal model of acute complete SCI in rats. DESIGN, TIME AND SETTING: A contrast control animal experiment was performed at the Center of Animal Experiment in College of Pharmaceutical, Shihezi University between November 2007 and October 2008. MATERIALS: Thirty healthy, Wistar rats were randomly divided into the control group (n=6), and the experimental group (n=24). METHODS: Rats in the experimental group were exposed processus spinosus and vertebral plate of T8-T12, and cut processus spinosus, and vertebral plate of T9-10 of corresponding spinal cord were served as injured region. The weight drop device was designed with the cerebrum three-dimensional positioning indicator based on Allen’s model. All of experimental animals were attacked T10 segment (15 g×20 cm=2.94×10-2 N). Be sure that the integrity and continuity of the dura in the experimental group were not damaged. There was no attack in the sham operation group. MAIN OUTCOME MEASURES: The motor function of both hind limbs were evaluated by oblique-plate test and BBB scores at 2, 4, and 8 weeks after operation. The pathological change of myeloid tissue was observed by hematoxylin-eosin staining. RESULTS: Rats in the sham operation group could walk after reviving, and the angle of oblique-plate test was more than 70°, with 21 scores. The micro-structure of spinal cord was normal. Rats in the experimental group were paralyzed completely after operation. Two rats showed spastic paralysis, and 5 rats showed different degree of self-mutilation. The angle of oblique-plate test was less than 30° with lower than 10 scores at weeks 2, 4, and 8 after operation. With the time increasing, some of rats had reflex activity to stimulation, but without active movement. The local inner-structure of spinal cord was damaged severely. CONCLUSION: An animal model of spinal cord injury with stable and complete can be established by attract with 2.94×10-2 N, which can ensure the integrity of spinal dura after operation.更多还原