【摘要】 背景:血清胆固醇水平增高与人群中骨关节病发病率增高呈正相关。而对于高糖高脂饮食是否参与关节软骨退变过程从而诱发骨关节炎,其参与机制又是什么,目前尚不清楚。目的:观察经高糖高脂喂养后新西兰兔膝关节软骨形态学改变,探索饮食在关节软骨退变过程中的作用。设计、时间及地点:随机对照动物实验,于2008-01/09在中南大学湘雅医学院动物实验室完成。材料:健康成年雄性新西兰兔40只,体质量约2kg。随机分为4组,每组10只。方法:①对照组饲以基础饲料作。②高糖组饲以高糖饲料(37%的蔗糖混合63%的普通饲料)。③高脂组饲以高脂饲料(20%猪油混合80%的普通饲料)。④高糖高脂组饲以高糖高脂饲料(37%蔗糖、10%猪油混合53%基础饲料)。主要观察指标:每4周末抽取空腹血样,测血糖、三酰甘油、总胆固醇及胰岛素水平,记录体质量变化。28周后取兔膝关节行大体观察后,取股骨髁部软骨行甲苯胺蓝染色、透射电镜观察。结果:①与对照组相比,高脂组及高糖高脂组血糖、三酰甘油、总胆固醇水平都显著升高,而胰岛素水平先升高后显著降低,上述变化均有显著性意义(P<0.05)。高糖组上述指标均较对照组升高,但差异无显著性意义(P>0.05)。②光镜和透射电镜下,高脂饮食组和高糖高脂饮食组可见潮线消失、软骨细胞排列紊乱、胶原纤维断裂、软骨细胞固缩等形态变化,高糖组未见明显改变。结论:长期高脂饮食及高糖高脂饮食可诱发和加重软骨损伤,提示其可能参与了骨关节炎的发生发展。更多还原

【Abstract】 BACKGROUND: There is a positive correlation between the serum cholesterol and osteoarthrosis incidence. However, it is still unclear whether the high-sugar and high-fat diet participates in the process of articular cartilage degeneration leading to osteoarthrosis, and the participation mechanism is unknown. OBJECTIVE: To investigate the changes in knee cartilage morphology of New Zealand white rabbits fed with high-sugar and high-fat diet and to explore the effect of high-sugar and high-fat diet on articular cartilage degeneration. DESIGN, TIME AND SETTING: The randomized controlled experiment was performed in Animal Laboratory, Xiangya Medical College from January to September 2008. MATERIALS: Forty healthy adult male New Zealand white rabbits, weighing about 2 kg, were randomly divided into 4 groups with 10 in each group. METHODS: Male New Zealand white rabbits were divided into four groups: ①normal diet group(NG), ②high-sugar diet (mixture of 37% cane sugar and 63% normal fodder) group(SG), ③high-fat diet (mixture of 20% lard and 80% normal fodder) group(FG), ④high-sugar and high-fat diet (mixture of 37% cane sugar, 10% lard and 53% normal fodder) group(SFG), MAIN OUTCOME MEASURES: Fasting blood samples were extracted every 4 weeks. The levels of blood sugar, triglyceride, cholesterol and insulin were detected, and body mass was recorded. The femoral condyle cartilage were dyed with toluidine blue, observed with transmit electron microscope after general observation of rabbit knee joints at the 28th weeks. RESULTS: ①Blood sugar, triglyceride, total cholesterol in FG and SFG were obviously higher than that in NG, the level of blood insulin in FG and SFG increased first, and then decreased significantly (P < 0.05).These indicators in SG were higher than those in NG, but the difference was not significant (P > 0.05). ② By light microscope and transmit electron microscope observation, the FG and SFG demonstrated that tide line disappeared, cartilage cells were ranged in disorder, collagen fibers fractured, cartilage cells and a change in shape, such as shrinkage. These changes of morphology were not observed in SG. CONCLUSION: Long-term high-fat diet or high-sugar and high-fat diet may induce or aggravate the disorder of articular cartilage, suggested that it may take part in the development of osteoarthritis.更多还原