【摘要】 目的:观察维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性与绝经后妇女骨密度及骨转换生化标志物的相关性。方法:①2007-01/2008-12从福州常住汉族人中随机检测绝经后妇女576例,年龄48～84(62.17±6.37)岁。受试者均知情同意。②记录年龄、绝经年限、体质量指数和绝经后骨折情况。③双能X射线骨密度仪检测正位第2～4腰椎、左侧股骨颈、大转子和Ward’s三角区骨密度。④PCR-RFLP技术检测维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性。⑤用酶联免疫吸附法检测骨转换生化标志物(血清骨钙素、血清骨碱性磷酸酶、尿吡啶啉和尿脱氧吡啶啉)。结果:561例合格受试者进入结果分析。①维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性各基因型间骨密度比较差异无显著性意义(P>0.05)。②维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性各基因型间骨转换生化标志物比较差异无显著性意义(P>0.05)。③维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性各基因型间骨质疏松症发生率比较差异无显著性意义(P>0.05)。④维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性各基因型间绝经后骨折发生率比较差异无显著性意义(P>0.05)。结论:维生素D受体基因BSMⅠ,TAQⅠ,APAⅠ多态性与绝经后骨质疏松症无明显关联,不能作为福州地区绝经后妇女骨质疏松的遗传标志。更多还原

【Abstract】 OBJECTIVE:To study the association of the vitamin D receptor gene BSMⅠ,TAQⅠ and APAⅠ genetic polymorphisms with bone mineral density and biochemical markers of bone turnover in postmenopausal women. METHODS:①A total of 576 postmenopausal Han ethnic women of 48-84 (62.17±6.37) years old in Fuzhou city were investigated,on the basis of their informed consent,through random sampling method from January 2007 to December 2008. ②The subjects were recorded regarding to their age,menopause duration,body mineral index and postmenopausal fracture incidence. ③ Dual energy X-ray absorptiometry was used for measuring the bone mineral density of vertebrae L2-4,left femoral neck,trochanter and Ward’s triangle. ④ The genetic polymorphisms of vitamin D receptor gene BSMⅠ ,TAQⅠ and APAⅠ were detected using polymerase chain reaction-restriction and fragment length polymorphism (PCR-RFLP) technique. ⑤The biochemical markers of bone turnover (serum bone gla protein,serum bone alkaline phosphatase,urinary pyridinoline and urinary deoxypyridinoline) were detected with enzyme linked immunosorbent assay. RESULTS:A total of 561 subjects up to standard were involved in the result analysis. ①T here was no significant difference in bone mineral density among genotypes of vitamin D receptor gene BSMⅠ ,TAQⅠ and APAⅠ polymorphisms (P > 0.05). ② There was no significant difference in the biochemical markers of bone turnover among genotypes of BSMⅠ ,TAQⅠ and APAⅠ polymorphisms (P > 0.05). ③ There was no significant difference in the incidence of osteoporosis among genotypes of BSMⅠ ,TAQⅠ and APAⅠ polymorphisms (P > 0.05). ④ There was no significant difference in the incidence of postmenopausal fracture among genotypes of BSMⅠ ,TAQⅠ and APAⅠ polymorphisms (P > 0.05). CONCLUSION:BSMⅠ ,TAQⅠ and APAⅠ polymorphisms of the vitamin D receptor gene are not obviously associated with osteoporosis in postmenopausal women,and accordingly can not be taken as genetic markers of osteoporosis in postmenopausal women in Fuzhou.更多还原