【摘要】 背景:有氧运动对于非酒精性脂肪性肝病有一定的预防和治疗作用,尚未见运动对于酒精性脂肪性肝病影响的报道。目的:观察中等强度运动对大鼠酒精性脂肪性肝病模型各种指标变化的影响,探讨运动对酒精性脂肪性肝病的作用。设计、时间及地点:随机对照动物实验,于2007-07/09在成都体育学院动物房完成。材料:4周龄健康雄性SD大鼠60只,实验分成3组,每组20只。方法:①空白对照组:注射等量生理盐水,不进行跑台运动训练。②模型对照组:给予乙醇+高脂饮食灌胃,放进静止的跑台不运动,0坡度,30min/d。③造模运动组:给予乙醇+高脂饮食灌胃,进行跑台运动训练,跑速25m/min,15%坡度,30min/d。运动持续4周。主要观察指标:检测各组肝脏组织中超氧化物歧化酶、丙二醛含量以及光镜观察组织学变化。结果:①空白对照组超氧化物歧化酶活性显著高于模型对照组和造模运动组(P<0.01),且前者超氧化物歧化酶达峰值。造模运动组超氧化物歧化酶活性高于模型对照组(P<0.05)。②空白对照组丙二醛含量显著低于模型对照组和造模运动组(P<0.01);造模运动组低于模型对照组(P<0.05)。模型对照组肝细胞变性程度显著重于空白对照组(P<0.01),造模运动组肝细胞脂肪变性程度轻于模型对照组(P<0.05),但仍然比空白对照组严重(P<0.01)。结论:适度的训练可维持机体自由基代谢正常进行,能够对酒精性脂肪性功能恢复产生促进作用,降低对肝脏的损伤。更多还原

【Abstract】 BACKGROUND:Aerobic exercise plays a positive role in the prevention and treatment of non-alcoholic fatty liver disease.As for alcoholic fatty liver disease,reports on it are still few.OBJECTIVE:To observe the moderate-intensity exercise-induced index changes of rat models with alcoholic fatty liver,so as to explore the effect of moderate-intensity exercise on alcoholic fatty liver disease.DESIGN,TIME AND SETTING:A randomized control animal trial was competed in the Animal Housing of Chengdu Sports University from July to September in 2007.MATERIALS:A total of 60 healthy male SD rats of 4-week old were divided into three groups in the experiment,with 20 ones in each group.METHODS:①Rats in the blank control group were injected with saline contour and they underwent no running training.②Rats in the model control group were fed with ethanol and pinguid food by intragastric gavage and were put on the static treadmill of 0 slope,with the frequency of 30 min/d.③Rats in the modeling exercise group were fed with ethanol and pinguid food by intragastric gavage,undergoing treadmill exercise at a speed of 25 m/min for 4 weeks,with the slope of 15% and the frequency of 30 min/d.MAIN OUTCOME MEASURES:The detected were contents of superoxide dismutases(SOD) and malonaldehydes(MDA) in the rat lever tissues.The histological changes were observed with histological light microscope.RESULTS:①The SOD activity in the blank control group was much higher than that eithther in the model control group or in the modeling exercise group(P<0.01),and the former one reached the peak value.The SOD activity in the modeling exercise group was higher than that in the model control group(P<0.01).② The MDA content in the blank control group was much lower than that either in the model control group or in the modeling exercise group(P<0.05);the MDA content in the modeling exercise group was lower than that in the model control group(P<0.05).Furthermore,the degree of liver cell degeneration of the model control group was significantly higher than that of the blank control group(P<0.01);the fatty degeneration of liver cells in the modeling exercise was lighter than that in the model control group(P<0.05),but still much severer than that in the blank control group(P<0.01).CONCLUSION:Appropriate training is capable of maintaining normal metabolism of free radicals in organisms,and can accordingly encourage the functional recovery of alcoholic fatty liver and limit its harm to levers.更多还原