【摘要】 背景:研究认为衰老和光老化状态下生长因子的表达不一定一致,生长因子与皮肤老化的关系越来越受到重视。目的:验证转化生长因子β1对长波紫外线照射体外培养的皮肤成纤维细胞胰岛素样生长因子1、角质细胞生长因子、血管内皮细胞生长因子表达的影响。设计、时间及地点:以体外培养细胞为观察对象,随机重复实验,于2006-07/2008-03在解放军第四军医大学全军整形外科研究所进行。材料:成纤维细胞来自解放军第四军医大学整形外科门诊患者包皮,采用组织块法培养获得。方法:通过酶联免疫法(ELISA)使用不同剂量长波紫外线(0,5,10,20J/cm2)照射体外培养的皮肤成纤维细胞,测定上清液中胰岛素样生长因子1、角质形成细胞生长因子、血管内皮细胞生长因子的质量浓度;以长波紫外线15J/cm2为照射剂量,选择不同剂量转化生长因子β1即小剂量组(0.1μg/L)、中剂量组(1μg/L)、大剂量组(10μg/L)对培养的皮肤成纤维细胞进行干预。主要观察指标:不同剂量长波紫外线照射、以及转化生长因子β1处理后皮肤成纤维细胞胰岛素样生长因子1、血管内皮细胞生长因子、角质形成细胞生长因子的表达。结果:长波紫外线照射体外培养的皮肤成纤维细胞导致胰岛素样生长因子1、角质形成细胞生长因子分泌水平下降,血管内皮细胞生长因子分泌升高;转化生长因子β1处理后,转化生长因子β1剂量依赖性地提高长波紫外线照射体外培养的皮肤成纤维细胞分泌的3种细胞因子的水平。转化生长因子β1大剂量组胰岛素样生长因子1、角质形成细胞生长因子、血管内皮细胞生长因子含量明显高于长波紫外线照射组(P<0.01)。结论:长波紫外线照射抑制体外培养成纤维细胞中胰岛素样生长因子1、角质形成细胞生长因子表达,促进血管内皮细胞生长因子表达。转化生长因子β1可提高长波紫外线照射体外培养的皮肤成纤维细胞分泌胰岛素样生长因子1、角质形成细胞生长因子、血管内皮细胞生长因子3种细胞因子的水平,有利于真皮内结构的恢复和重建。更多还原

【Abstract】 BACKGROUND:Studies demonstrates that aging is not completely consistent with the expression of growth factor under photoaging,but the relation between growth factor and skin aging has attracted more attention. OBJECTIVE:To investigate the effects of transforming growth factor beta 1(TGF-β1) on the expression of insulin-like growth factor 1(IGF-1) ,keratinocyte growth factor(KGF) ,and vascular endothelial cell growth factor(VEGF) after ultraviolet(UVA) irradiation. DESIGN,TIME AND SETTING:A randomized repeated experiment based on cultivate cells was performed at the Key Laboratory,Institute of the Plastic Surgery,Fourth Military Medical University from July 2006 to March 2008. MATERIALS:Fibroblasts were harvested from the prepuce of patients of Department of Outpatient,Institute of the Plastic Surgery,Fourth Military Medical University and cultured with tissue block method. METHODS:Skin fibroblasts were irradiated with different doses of UVA(0 J/cm2,5 J/cm2,l0 J/cm2,20 J/cm2) ,and the mass concentration of IGF-1,KGF,and VEGF in the supernatant was measured by enzyme-linked immunosorbent assay(ELISA) . UVC irradiation dose was 15 J/cm2. TGF-β1 with low,medium and high dose(0.1 μg/L,1.0 μg/L,10.0 μg/L,especially) was added to irradiate the fibroblasts. MAIN OUTCOME MEASURES:The expression of IGF-1,KGF,as well as VEGF after irradiated with different doses of UVA. RESULTS:The secretion level of IGF-1,KGF was decreased,and VEGF was increased in cultured skin fibroblasts after UVA irradiation. The levels of IGF-1,KGF and VEGF were increased after adding TGF-β1,which showed a dose-dependent manner. Contents of IGF-1,KGF,and VEGF in the large dose group were significantly higher than those of the UVA irradiation group(P < 0.01) . CONCLUSION:In vitro UVA can inhabit the expression of IGF-1 and KGF in skin fibroblasts,meanwhile,can increase VEGF expression. TGF-β1 can elevate levels of IGF-1,KGF,and VEGF secreted by UVA irradiated dermal fibroblasts,which in favor of the restore and reestablishment of dermal structures.更多还原