【摘要】 背景:温度敏感性磁性纳米颗粒即磁性纳米颗粒-Pluronic具有温度响应药物控制释放能力,并能穿透血脑屏障。目的:实验检测其携带神经节苷脂(GM-1)能力及在活体组织中药物释放能力,以及对脊髓损伤修复再生的影响。设计、时间及地点:随机对照动物实验,于2006-09/2007-02在首都医科大学北京神经科学研究所和中国科学院过程工程研究所完成。材料:神经节苷脂由阿根廷Trb Pharma药厂生产。磁性纳米颗粒-Pluronic的制备及其负载神经节苷脂由中国科学院过程工程研究所制备。方法:将20只SD大鼠制作急性完全横断脊髓损伤模型,随机分为4组:①治疗组:于脊髓断端间注射磁性纳米颗粒-Pluronic-GM-1100μL+纤维蛋白胶100μL。②对照组1:于脊髓断端间注射磁性纳米颗粒-Pluronic100μL+纤维蛋白胶100μL。③对照组2:于脊髓断端间注射纤维蛋白胶100μL。④空白组:单纯横断不予以任何治疗。主要观察指标:4周后,应用免疫组织化学方法结合图像分析方法,对脊髓横断处远端、近端进行神经纤维计数及胶质细胞网格框架结构定量分析,以了解神经再生情况。结果:20只大鼠均进入结果分析。①脊髓横断区神经纤维数量:治疗组近端、远端均高于其他3组(P<0.05),2个对照组高于空白组(P<0.05),对照组2高于对照组1(P<0.05)。②脊髓横断区胶质纤维面积比:治疗组近端、远端均高于其他3组(P<0.05),2个对照组近端明显高于空白组(P<0.05),对照组2远端高于空白组(P<0.05)。结论:磁性纳米颗粒-Pluronic具有药物携带及释放作用,其携带神经节苷脂对脊髓损伤具有一定程度的修复及促进神经再生的作用。更多还原

【Abstract】 BACKGROUND:Temperature-responsive magnetic pluronic nanoparticles possess the capacity of drug release controlled by body temperature and can penetrate blood brain barrier. OBJECTIVE:To detect the capacity of magnetic pluronic nanoparticles to carrying ganglioside-1(GM-1) and its capacity of drug release in vivo,as well as its effects on repair of spinal cord injury. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the key laboratories of Beijing Institute for Neuroscience,Capital Medical University and Institute of Process Engineering,Chinese Academy of Sciences between June 2006 and February 2007. MATERIALS:GM-1 loaded magnetic pluronic nanoparticles were prepared by Laboratory of Separation Science and Engineering State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences. GM-1 was provided by Trb Pharma S.A of Argentina. METHODS:Twenty Sprague-Dawley rats were developed into acute complete spinal cord injury models and were then evenly and randomly divided into four groups:①GM-1 loaded magnetic pluronic nanoparticles + fibrin glue(treatment group) :100 μL GM-1 loaded magnetic pluronic nanoparticles and 100 μL fibrin glue injected into the incision. ②Magnetic pluronic nanoparticles+fibrin glue(control group 1) :receiving 100 μL magnetic pluronic nanoparticles and 100 μL fibrin glue. ③ Fibrin glue(control group 2) :administration of 100 μL fibrin glue. ④ Sham-operation group:no treatments. MAIN OUTCOME MEASURES:Behavior evaluation and quantitative analysis(nerve fibers and glial cells) at 4 weeks post-surgery. RESULTS:All initial 20 rats were included in the final analysis. ① The numbers of nerve fibers in the rostral and caudal areas were significantly greater in the treatment group than in the remaining 3 groups(P < 0.05) ,the control groups exhibited greater numbers of nerve fibers than the sham-operation groups,and the control group 2 had higher levels compared with the control group 1(P < 0.05) . ②The area ratio of glial cells in the rostral and caudal areas was significantly higher in the treatment group compared with the remaining 3 groups(P < 0.05) . In the rostral areas,the control groups exhibited higher area ratio of glial cells than the sham-operation group(P < 0.05) ,and in the caudal areas,control group 2 displayed higher levels compared with the sham-operation group(P < 0.05) . CONCLUSION:Magnetic pluronic nanoparticles possess drug-carrying and drug-releasing capacities. GM-1 loaded magnetic pluronic nanoparticles can repair spinal cord injury and promote nerve regeneration.更多还原