【摘要】 背景:血管内皮细胞生长因子和转化生长因子β1都是与骨关节炎关系比较密切的合成及分解类细胞因子家族,在骨关节炎组织中表达情况的报道多限于软骨细胞及基质方面,且报道结果并不完全一致,对滑膜组织研究尚不深入。目的:进一步了解血管内皮细胞生长因子和转化生长因子β1在骨关节炎滑膜组织中的表达及相关性。设计、时间及地点:细胞分子生物学体外实验,2003-01/2004-09在滨州医学院附属淄博市中心医院分子生物学实验室完成。对象:选取符合骨关节炎诊断标准并经辅检证实的患者的滑膜病理标本30例;非正常死亡及创伤性截肢的健康国人滑膜标本8例为正常对照。方法:所有滑膜标本进行免疫组织化学染色,常规脱蜡至水,先后加入过氧化酶阻断溶液及正常羊血清孵育,并依次加一抗、生物素标记二抗、链亲和素-过氧化物酶溶液及DAB溶液,其间均充分水洗,苏木精复染封固观察。对照实验采用替代实验,用PBS替代一抗,余步骤同上述实验方法。主要观察指标:血管内皮细胞生长因子和转化生长因子β1在滑膜组织中的表达情况及其相关性。结果:血管内皮细胞生长因子和转化生长因子β1的阳性表达率分别为86.7%和13.3%,与正常组织比较,转化生长因子β1表达率降低(P<0.05),血管内皮细胞生长因子表达率增高(P<0.05)。血管内皮细胞生长因子和转化生长因子β1表达的相关性有显著性意义(rs=0.373,0.01<P<0.05)。结论:血管内皮细胞生长因子和转化生长因子β1在骨关节滑膜组织中的表达有相关性。血管内皮细胞生长因子和转化生长因子β1的改变是骨关节炎发生、发展的重要介导因素。更多还原

【Abstract】 BACKGROUND: The vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) are the family members of the synthesis and decomposition class cell factors, which closely relate to osteoarthritis. But the expression of VEGF and TGF-β1 in previous reports mainly focuses on the cartilage cell and matrix aspect in osteoarthritis is superficial, especially in synovial membrane tissues. OBJECTIVE: To further understand the expression of VEGF and TGF-β1 in the synovial membrane of osteoarthritis, and to study the relationship between the two factors. DESIGN, TIME AND SETTING: The in vitro cytology experiment was completed in the Molecular Biology Laboratory of Zibo Central Hospital, Affiliated Hospital of Binzhou Medical College from January 2003 to September 2004. PARTICIPANTS: A total of 30 pathology specimen of synovial membrane which conformed to the diagnoses standard of osteoarthritis, and 8 pathology specimen of synovial membrane which were unnatural death or traumatic amputation healthy people were selected. METHODS: All synovial membrane specimen were performed immunohistochemistry staining, peroxidase solution, normal horse serum, first antibody, polyvalent biotinylated antibody, streptavidin-peroxydase solution, and DAB solution were added orderly, then followed by haematoxylin staining. In the control group, all procedure was the same as the synovial membrane instead of the first antibody was replaced with PBS. MAIN OUTCOME MEASURES: The expression of VEGF and TGF-β1 in the synovial membrane and their relationship. RESULTS: The positive expression rates of VEGF and TGF-β1 were 86.7% and 13.3%, respectively. Compared with normal tissues, the positive expression rate of TGF-β1 was lower (P < 0.05), while, the positive expression rate of VEGF was increased (P < 0.05). The correlation coefficient between VEGF and TGF-β1 had statistical significance (rs=0.373, 0.01 < P < 0.05). CONCLUSION: There had correlation between the expression of VEGF and TGF-β1 in the synovial membrane of osteoarthritis. The changes of VEGF and TGF-β1 are the important mediating factors in occur and development of osteoarthritis.更多还原