【摘要】 目的探讨夏枯草提取物对α-淀粉酶的抑制作用。方法采用水提和水提醇沉方法得到夏枯草水提物、浸膏、粗多糖,通过Van Loon′s碘比色法检测α-淀粉酶活性,以夏枯草水提物测定该酶动力学。结果夏枯草水提物对α-淀粉酶有抑制作用,呈量效关系,对该酶抑制类型为反竞争性抑制。夏枯草浸膏对α-淀粉酶抑制作用较水提物明显。结论夏枯草水提物、浸膏对α-淀粉酶存在抑制作用,有效部位可能在浸膏部分,为深入研究夏枯草提取物抑制单糖吸收作用提供了依据。更多还原

【Abstract】 Objective It is to explore the inhibiting function of extract from Prunella vulgaris L.on α-amylase(α-AL).Methods Aqueous extract, polysaccharide and aqueous-ethanol extract was obtained from Prunella vulgaris L.by aqueous extract and ethanol extract methods.The activity of α-AL was determined through Van Loon′s method,using aqueous extract from Prunella vulgaris L.to determine its enzyme kinetics.Results Aqueous extract from Prunella vulgaris L.could inhibit the activity of α-AL,and there was a dose-effect relationship between them.The inhibiting type of this amylase was uncompetitive inhibition.The effect of aqueous extract from Prunella vulgaris L.on α-AL was more obvious than that of aqueous-ethanol extract.Conclusion Aqueous extract and aqueous-ethanol extract from Prunella vulgaris L.has inhibiting effect on α-AL,and the utility part of Prunella vulgaris L.was aqueous-ethanol extract,which provides an evidence to do further study on inhibiting glucose absorption of Prunella vulgaris L.更多还原