【摘要】 目的:观察针刺"内关""足三里"对心肌缺血模型大鼠孤束核(nucleus of the solitary tract,NTS)c-fos表达及心电图STⅡ的影响,阐明延髓初级中枢在"内关""足三里"穴共同调节心功能中的作用。方法:将36只雄性SD大鼠随机分为生理盐水组、模型组、足三里组、内关组、偏历组及合阳组,每组6只,腹腔注射异丙肾上腺素造成心肌缺血模型。电针治疗20 min,采用抗Fos蛋白免疫组织化学技术观察大鼠NTS内c-fos表达和电生理技术观察大鼠心电图STⅡ的变化。结果:心肌缺血模型大鼠NTS内c-fos的表达明显增加(P<0.01),而足三里组、内关组与模型组比较,NTS内c-fos阳性细胞数均明显减少(P<0.01);足三里组、内关组NTS内c-fos阳性细胞数显著低于偏历组、合阳组(P<0.01)。足三里组、内关组大鼠缺血性心电图STⅡ电位值显著降低(P<0.01),其电位降低值大于偏历组、合阳组(P<0.01)。结论:NTS是针刺内关、足三里共同调节心功能的整合中枢之一。更多还原

【Abstract】 Objective To observe the effect of electroacupuncture(EA) of "Neiguan"(PC 6) and "Zusanli"(ST 36) on c-fos expression in the nucleus of the solitary tract(NTS)and ischemic electrocardiogram(ECG) in acute myocardial ischemia(AMI) rats,so as to study the role of NTS in EA-induced improvement of AMI.Methods Thirty-six male SD rats were randomly divided into control,model,ST 36,PC 6,"Pianli"(LI 6),and "Heyang"(BL 55) groups,with 6 cases in each.AMI model was established by intraperitoneal injection of Isoproterenol(5 mg/kg).EA(4 Hz/20 Hz,1 mA) was applied to PC 6,ST 36,LI 6 and BL 55 for 20 min separately.c-fos expression in the NTS was detected by immunohistochemistry and ECG-STⅡ was recorded before and after AMI and EA.Results ① Compared to control group,ECG-STⅡ of model group elevated significantly,while in comparison with model group,ECG-STⅡ values of ST 36 and PC 6 groups decreased significantly(P<0.01),suggesting an improvement of AMI after EA.No significant changes of ECG-STⅡ were found in LI 6 and BL 55 groups(P>0.05).② The number of fos-immunoreaction(fos-IR) positive neurons in the NTS in ST 36 and PC 6 groups was significantly lower than that in model,LI 6 and BL 55 groups(P<0.01).Conclusion EA of PC 6 and ST 36 can improve ischemic ECG in AMI rats and NTS is one of the centers for integrating cardiac functional activity and information from EA of PC 6 and ST 36.更多还原