【摘要】 目的:明确黄连提取物及其化学成分对豚鼠胃平滑肌收缩运动的影响,探讨与黄连"健胃"和"败胃"作用相关的药效物质基础。方法:采用张力测定法,观察黄连水提物、黄连总生物碱(0.3～1 000μg/mL)及其化学成分小檗碱、巴马丁碱和药根碱(0.3～1000μmol/L)对豚鼠离体胃窦环行肌自发收缩和电场刺激(electrical field stimulation,EFS)诱发收缩的影响。结果:小剂量黄连水提物和黄连总生物碱均能促进胃窦环行肌的自发收缩,而大剂量则抑制。小檗碱、巴马丁碱和药根碱具有相似效应,其中在发挥促收缩作用时药根碱作用最强,在发挥抑制作用时小檗碱作用最强。另外,黄连水提物、黄连总生物碱、小檗碱、巴马丁碱和药根碱均能增加EFS诱发的平滑肌收缩幅度,其中药根碱的作用最强。结论:小剂量黄连"健胃"与大剂量"败胃"作用可能与其影响胃平滑肌收缩有关。小檗碱、巴马丁碱和药根碱都是黄连影响胃平滑肌收缩的有效成分,药根碱促平滑肌收缩的作用最强,而小檗碱抑制平滑肌收缩的作用最强。更多还原

【Abstract】 Objective:To identify the influence of extracts and active components of Rhizoma Coptidis on gastric smooth muscle contractility of guinea pigs,and to explore the potential pharmacological mechanism of Rhizoma Coptidis in "invigorating the stomach" and "impairing the stomach". Methods:Observing the effects of the water extract and the alkaloids from Rhizoma Coptidis (at doses ranging from 0.3 to 1 000 μg/L) and other active components such as berberine,palmatine and jatrorrhizine (at doses ranging from 0.3 to 1 000 μmol/L) on the spontaneous and electrical field stimulation (EFS)-induced contractions of antral circular smooth muscle strips from guinea pig stomach via a force transducer in vitro. Results:The water extract or the alkaloids from Rhizoma Coptidis could improve the spontaneous contraction at the low doses,but inhibit the spontaneous contraction at the high doses.Berberine,palmatine and jatrorrhizine also showed the similar effects.Moreover,the water extract and the alkaloids of Rhizoma Coptidis,as well as berberine,palmatine and jatrorrhizine could increase the EFS-induced contraction. Among the three monomers,jatrorrhizine exhibited the most potent effect on EFS-induced contraction. Conclusion:The effects of Rhizoma Coptidis in "invigorating the stomach" or "impairing the stomach" may be related to its effect on gastric smooth muscle contractility.Berberine,palmatine and jatrorrhizine are all effective components of Rhizoma Coptidis affecting the contraction of gastric smooth muscle,among which jatrorrhizine is the most potent agent in promoting the contraction while berberine is the most potent one for inhibiting the contraction.更多还原