【摘要】 目的:研究益气化瘀补肾方治疗气虚血瘀肾虚型颈椎病大鼠的作用机制。方法:选择3月龄雌性SPF级SD大鼠30只,随机分为正常组、模型组和益气化瘀补肾方组,每组10只。气虚血瘀肾虚型颈椎病模型通过病、证模型复合而成。益气化瘀补肾方组采用益气化瘀补肾方治疗1个月。通过放射免疫学、组织病理学、免疫组织化学和分子生物学等技术研究益气化瘀补肾方的作用机制。结果:与正常组比较,模型组动物出现精神萎靡、少动,舌质、尾色瘀紫,体质量减轻,子宫及附件质量降低,环磷酸腺苷与环磷酸鸟苷及其比值无明显差异,血黏度、血小板表面α-颗粒膜糖蛋白(alpha-granular mem-brane protein,CD62p)增高,血清雌二醇(estradiol,E2)降低。益气化瘀补肾方组与模型组比较,除子宫及附件质量外,上述指标均有不同程度改善。HE染色显示模型组颈椎间盘高度降低,纤维环出现裂隙,胶原纤维排列紊乱,髓核减少,软骨终板变薄;益气化瘀补肾方组椎间盘高度正常,纤维环排列稍紊乱,髓核结构良好,软骨终板变化不明显。与正常组比较,模型组Ⅱ型胶原表达减少;益气化瘀补肾方组Ⅱ型胶原在纤维环的表达比模型组有所增加。模型组与正常组比较Ⅱ型前胶原基因和基质金属蛋白酶抑制剂-1(tissuein-hibitor of metalloproteinase-1,TIMP-1))基因表达降低(P<0.01),益气化瘀补肾方组与模型组比较,Ⅱ型前胶原基因和TIMP-1基因表达增高,基质金属蛋白酶-13基因表达降低(P<0.05,P<0.01)。结论:益气化瘀补肾方通过调节免疫、凝血和内分泌系统等改善模型动物气虚血瘀肾虚状态,通过调节椎间盘细胞外基质以及相关金属蛋白酶延缓椎间盘退变。更多还原

【Abstract】 Objective:To explore the mechanism of Yiqi Huayu Bushen Recipe(YHBR),a compound traditional Chinese herbal medicine,in treating cervical syndrome(CS) with qi deficiency,blood stasis and kidney deficiency in rats.Methods:A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group,untreated group and YHBR group;there were ten rats in each group.The rat model of cervical syndrome with qi deficiency,blood stasis and kidney deficiency was established by combining disease and syndrome models.After one-month YHBR treatment in YHBR group,all rats were sacrificed.Serum,plasma and cervical intervertebral discs were detected and observed by radio-immunology,histopathology,immunohistochemistry,etc.Results:Compared with those in the normal control group,rats in the untreated group showed obvious signs of deficiency in vital energy,such as tiredness,ptosis,few movement,bluish-purple tongue and tail and weight loss;the weight of uterus and appendages,and the ratio of cyclic adenosine monophosphate/cyclic guanosine monophosphate were decreased;hemorheological parameters and the expression of alpha-granular membrane protein(CD62p) were increased;the content of serum estradiol was decreased.YHBR could improve the above indexes except for the weight of uterus and appendages.HE staining showed annulus fibrosus with cracks,diminish of pulposus nucleus,and decrease in the height of intervertebral disc and the thickness of end-plate in the untreated group;and YHBR could improve the changes of cervical intervertebral discs,but no obvious changes in end-plate.Type Ⅱ collagen protein(Col2a1)was distributed in every part of the annulus fibrosus in normal control group,but was decreased in untreated group;YHBR could increase Col2a1 expression in annulus fibrosus as compared with the untreated group.The expressions of Col2a1 and tissue inhibitor of metalloproteinase-1(TIMP-1) mRNAs were decreased in untreated group as compared with the normal control group(P<0.01);the expressions of Col2a1 and TIMP-1 mRNAs were higher and matrix metalloproteinase-13 mRNA was lower in the YHBR group than those in the untreated group(P<0.05,P<0.01).Conclusion:YHBR may improve the condition of cervical syndrome with qi deficiency,blood stasis and kidney deficiency by regulating immune system,coagulation system and endocrine system,and delay the degeneration of cervical intervertebral disc by regulating extracellular matrix and metalloproteases in intervertebral disc.更多还原