【摘要】 目的研究分析昆明市2006年注射吸毒者中艾滋病病毒-1型(HIV-1)的基因亚型流行情况,为更清楚地掌握昆明市HIV流行模式,制订抗病毒治疗方案和疫苗研发提供参考数据。方法采用"一对一"的问卷调查,收集基本人口学特征及HIV感染危险行为信息,采集血样,利用套式聚合酶链反应(Nested-PCR)对HIV前病毒的gag基因进行扩增,对扩增产物进行纯化、测序,通过构建系统进化树和在线比对来判定序列所属基因亚型。结果62名研究对象所感染HIV-1gag基因被成功扩增、测序、判定基因亚型。其中1人所感染的HIV-1为CRF01-AE重组型,其余均为CRF07-BC重组型或CRF08-BC重组型。12名对象除注射海洛因外,还发生过商业性行为,感染CRF01-AE的对象就是发生过商业性行为的注射吸毒者。结论2006年发现的HIV-1阳性注射吸毒者中,CRF07-BC、CRF08-BC重组型毒株占据了绝对优势,同时存在CRF01-AE的流行。更多还原

【Abstract】 Objective To identify human immunodeficiency virus type 1 (HIV-1) subtypes among local HIV-1 positive injecting drug users (IDUs) reported in 2006 in Kunming,the capital city of Yunnan province,and provide reference for understanding HIV epidemic,establishing protocol of ART and developing vaccine.Methods After oral informed consent,HIV-1 positive IDUs reported in Kunming in 2006 were interviewed to collect their personal information and HIV-related risk behaviors,their blood was collected and DNA was extracted.After amplification of HIV-1 gene fragment by nested polymerase chain reaction,gag sequences were determined,purified and sequenced,HIV-1 subtype was therefore identified by analyzing phylogenetic tree and on-line analysis through Los Alamos HIV database.Results HIV-1 subtypes in 62 HIV-1 positive IDUs reported in 2006 in Kunming were identified,and 1 case was CRF01-AE and the other cases were CRF07-BC or CRF08-BC.Among all these HIV-1 positive IDUs,12 had commercial sex behaviors and the one who was infected with CRF01-AE was among these 12 subjects.Conclusion CRF07-BC or CRF08-BC has taken an absolutely predominate position in HIV-1 positive IDUs reported in 2006 in Kunming,and CRF01-AE is also circulating,which makes HIV-1 prevalence in Kunming more complicated.更多还原