【摘要】 目的从基因水平了解北京艾滋病病毒(HIV)抗体阳性的男男性行为者人群(MSM)中,人类白细胞抗原(HLA)-A、-B、-DRB1位点的等位基因频率,分析其与HIV感染者自身病毒载量的关系。方法应用聚合酶链反应-序列特异性引物技术(PCR-SSP),对北京32例HIV阳性MSM进行了HLA-A、-B、-DRB1等位基因分型。结果鉴定了12个HLA-A等位基因,21个HLA-B等位基因,12个HLA-DRB1等位基因。最常见的等位基因分别为A*02(26.56%)、B*40(17.19%)和DRB1*15(20.31%)。含有A*02等位基因组者的血浆病毒载量,较不含有此等位基因组者低(P=0.002),而含有HLA-B*40和HLA-DRB1*15等位基因组者的血浆病毒载量,较不含有对应等位基因组者高(HLA-B*40:P=0.799;HLA-DRB1*15:P=0.021)。结论北京HIV阳性MSM人群HLA-A、-B、-DRB1基因多态性较高。HLA-A*02等位基因在该人群中可能与延缓AIDS疾病进程相关,而HLA-DRB1*15等位基因可能与加速该人群AIDS疾病进程相关。更多还原

【Abstract】 Objective To explore allele frequency of HLA-A,HLA-B,HLA-DRB1 among HIV-positive men who have sex with men (HIV+MSM) in Beijing and to analyze its correlation with viral load in plasma.Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-A,HLA-B and HLA-DRB1 alleles among 32 HIV+MSM in Beijing.Result 12 A,21 B and 12 DRB1 alleles were detected.The most common HLA-A,HLA-B and HLA-DRB1 allele was A02,B40,DRB115 with the frequency of 26.56%,17.19% and 20.31%,respectively.The plasma viral load of individuals with HLA-A02 alleles was lower than in those without HLA-A02 alleles (P=0.002),however,the plasma viral load in individuals with either HLA-B40 or HLA-DRB115 was higher than in those without HLA-B40 or HLA-DRB115,(HLA-B40,P=0.799,HLA-DRB115,P=0.021).Conclusion The polymorphisms of HLA-A,-B,and-DRB1 loci are high,HLA-A02 alleles may be associated with slower HIV disease progression,whereas HLA-DRB115 alleles are potentially associated with faster disease progression among HIV-positive MSM in Beijing.更多还原