【摘要】 目的探讨艾滋病病毒Ⅰ型(HIV-1)感染者CD4+T淋巴细胞(CD4细胞)及其各细胞亚群:纯真细胞(TN)、中心记忆细胞(TCM)和效应记忆细胞(TEM)的增殖和消亡情况。方法观察23例未经抗病毒治疗、感染时间大于12个月的HIV-1感染者,以16例健康者为对照,通过流式细胞仪分析CD4+T淋巴细胞CD45RO和CD27的表达情况,把CD4细胞分为TN(CD45RO-CD2+7)、TCM(CD45RO+CD2+7)和TEM(CD45RO+CD2-7)三个亚群。以Ki-67抗原的表达代表细胞的增殖,AnnexinV检测阳性代表细胞的消亡,比较HIV-1感染组和健康组CD4细胞及其各个亚群增殖和消亡的情况。结果健康组(n=16例)CD4细胞增殖率为(0.36±0.24)%,各亚群增殖率分别为TN(0.07±0.05)%,TCM(0.57±0.52)%和TEM(0.40±0.39)%;CD4细胞消亡率为(6.30±2.41)%,各亚群消亡率分别为TN(4.43±2.21)%,TCM(6.58±2.50)%和TEM(12.47±8.98)%;与之相比,HIV-1感染组(n=23例):CD4细胞增殖率为(2.93±2.72)%(z=-4.511,P=0.000),各亚群增殖率:TN(0.52±0.42)%(z=-4.785,P=0.000),TCM(5.55±5.07)%(z=-4.241,P=0.000)和TEM(4.53±5.20)%(z=-3.884,P=0.000);CD4细胞消亡率为(23.59±16.27)%(z=-3.883,P=0.000),各亚群消亡率:TN(17.25±15.80)%(z=-2.227,P=0.026),TCM(25.16±16.55)%(z=-3.940,P=0.000),TEM(29.56±16.77)%(z=-3.626,P=0.000)。与健康组相比,HIV-1感染组CD4及其各细胞亚群增殖率和消亡率明显增加,差异有统计学意义。结论与健康组相比,HIV-1感染者CD4细胞及其各个亚群细胞消亡和增殖有明显增加,细胞进入高速更新的状态,纯真细胞亚群和记忆细胞亚群组成比没有明显变化。更多还原

【Abstract】 Objective To observe the changes in proliferation and death of CD4+ T cell(TCD+4)and its subsets including naive CD+4 cell(TN),central memory CD+4 cell(TCM)and effect memory CD+4 T cell(TEM)in HIV-1 infected patients.Methods Sixteen healthy controls and 23 untreated HIV-1 infected patients whose infection duration was over 12 months were enrolled for observation.Flow cytometry tests were carried out after routine staining.CD+4 T cells were divided into three subsets based on CD45RO and CD27 expressions.They were TN(CD45RO-CD+27),TCM(CD45RO+CD+27),and TEM(CD45RO+CD-27)respectively.Cell proliferation was indicated by Ki-67 antigen expression.Cell death was indicated by positive Annexin V stain.Cell proliferation and death rates were compared between the HIV-1 infected group and the healthy control group.Results The CD+4 T cell proliferation rates in the healthy controls were TCD4+ 0.36%±0.24%,TN0.07%±0.05%,TCM0.57%±0.52%,and TEM0.40%±0.39% respectively.The death rates in the healthy controls were TCD4+6.30%±2.41%,TN4.43%±2.21%,TCM6.58%±2.50%,and TEM12.47%±8.98% respectively.The proliferation rates in the HIV-1 infected group were TCD4+ 2.93%±2.72%(z=-4.511,P=0.000),TN0.52%±0.42%(z=-4.785,P=0.000),TCM5.55%±5.07%(z=-4.241,P=0.000),TEM4.53%±5.20%(z=-3.884,P=0.000);the,death rates in this group were TCD4+ 23.59%±16.27%(z=-3.883,P=0.000),TN17.25%±15.80%(z=-2.227,P=0.026),TCM25.16%±16.55%(z=-3.940,P=0.000),and TEM29.56%±16.77%(z=-3.626,P=0.000).Compared with the normal controls,the proliferation and death rates in the HIV-1 infected group were dramatically increased and the changes had statistic difference.Conclusions Compared with the normal controls,the proliferation and death of CD+4 T cell and its subsets in the HIV-1 infected group were dramatically increased.CD+4 T cells were shifted into high flow-rate cell cycling.The compositive ratios of naive CD+4 cell subset and memory CD+4 cell subset were unchanged in HIV-1 infected group.更多还原