【摘要】 【目的】研究双酚A(BPA)对小鼠肾组织的毒性效应。【方法】将BPA溶于金龙食用油中,备用。将20只小鼠随机分为5组:Ⅰ组为对照组,注射食用油1 mL/只;Ⅱ~Ⅴ组为药物处理组,分别注射0.1,0.5,1或2mg/mL的BPA 1 mL/只,每5 d注射1次,连续注射3次。于末次注射后第5,10,15和20天各组分别取1只小鼠处死,取肾脏,用多聚甲醛固定,常规方法制备切片,免疫组织化学染色显微观察-βactin的分布定位,分析BPA对小鼠肾组织-βactin阳性细胞表达的影响。【结果】第5~15天,0.1和0.5 mg/mL BPA处理组小鼠肾组织-βactin阳性细胞数量与对照组差异不显著,1和2 mg/mL BPA处理组小鼠肾组织阳性细胞数量与其他3组差异显著;第20天,各BPA处理组小鼠-βactin阳性细胞数量与对照组差异显著。【结论】小鼠肾组织中-βactin阳性细胞随BPA剂量的增加与用药时间的延长而减少。更多还原

【Abstract】 【Objective】 The toxicity of intraperitoneal injection of bisphenol A(BPA) of different doses to kidney tissue of mice was evaluated with the change of β-actin expression.【Method】 BPA was resolved in Jinlong plant oil.20 mice were divided into 5 groups.One group injected with the plant oil was used as control.The other groups were injected with different doses of BPA(0.1,0.5,1 or 2 mg/mL per mice) at intervals of 5 d for 3 times respectively.After the injection of BPA or oil for 5,10,15 and 20 d,the mice were killed respectively.Then,each mouse kidney tissue was removed and fixed in polyformaldehyde.The change of β-actin expression kidney tissue was observed by immunohistochemical staining.【Result】 Compared with control group,after treatment with BPA from 5 d to 15 d,β-actin expression showed no change with the lower dose of BPA at 0.1 and 0.5 μg/mL.However,it was decreased significantly with the higher dose of BPA at 1 and 2 mg/mL.After the treatment with BPA for 20 d,the β-actin expression in all of groups treated with BPA was reduced greatly.【Conclusion】 These results indicated that the number of β-actin-stained positive kidney cells was reduced by bisphenol A at a dose-dependent and time-dependent manner,and thus the systematic bisphenol A showed the strong toxicity of mice kidney tissue.更多还原