【摘要】 为了提高小麦的耐逆性,采用穗茎注射法将反义PLDγ基因导入小麦品种LK906中。通过卡那霉素筛选获得了91株抗性植株。提取抗性植株叶片DNA进行PCR扩增,结果有42株扩增出430 bp目的条带,PCR-Southern杂交也证实其为阳性。RT-PCR检测发现,反义PLDγ基因在10株植株已经表达。同时试验发现,转基因小麦的耐盐性与对照相比均达到极显著水平。从分子水平及性状表现说明目的基因已经转入受体小麦中且能够成功表达。更多还原

【Abstract】 Phospholipase Dγ is an enzyme which can affect penetrability of cell membrane.Researches showed that the plants` stress tolerance can be enhanced by Antisense phospholipase Dγ(anti-PLDγ).In this experiment,anti-PLDγ was introduced into LK906 wheat mediated by earstem injecting of Agrobacterium.Based on kanamycin selection,91 plants were obtained.The transformants were screened by PCR,PCR-southern and RT-PCR technique.Ten transgenic plants were obtained.At the same time,it was observed that salt stress tolerance of transgenic plants was enhanced highly.The result was that the gene can be expressed after introduced into wheat.更多还原