【摘要】 为探讨适用于小鼠子宫内膜上皮细胞的培养方法,对胰蛋白酶消化法、胰蛋白酶消化+刮取法、Ⅰ型胶原酶消化法、Ⅰ型胶原酶消化法+刮取法、子宫翻转组织块培养法5种方法进行了比较研究。结果表明,胰蛋白酶消化法获得的上皮细胞数量稀少,活力低,生长速度缓慢;刮取法+胰蛋白酶消化法、胶原酶消化法、刮取法+Ⅰ型胶原酶消化法获得的细胞数量较单纯胰蛋白酶消化法多,但达不到研究所需的要求;子宫翻转组织块培养法获得的上皮细胞活力强,生长速度快,纯度高,比较适宜小鼠子宫内膜上皮细胞的原代培养。更多还原

【Abstract】 To study the suitable method for mouse endometrial epithelial cell culture,five methods were compared: trypsin digestion,trypsin digestion + shaving,collagenase Ⅰdigestion,collagenase Ⅰ digestion + shaving,turned over uterus tissue culture.The result indicated that cell is small,vigor is low and grows slowly by the method of trypsin digestion.By the method of trypsin digestion + shaving,collagenase Ⅰdigestion and collagenase Ⅰ digestion + shaving,the number of cell is bigger than trypsin digestion,but they cann’t reach the standard also.The last method of turned over uterus tissue culture is best for mouse endometrial epithelial cell culture.By this method,the cell vigor is high,cell grows quickly and purely.更多还原