【摘要】 以胚龄(Ed)45~240牦牛上睑皮肤为材料,同一个体背部皮肤作为对照,通过组织切片HE染色和免疫组化方法研究睫毛毛囊的形态发生过程,检测Shh和β-catenin在牦牛睫毛毛囊形态发生中的时空表达和互作规律。结果表明,在睫毛毛囊的形态发生中表皮-间充质相互作用以表皮为主导间充质随着表皮而变化,β-catenin从基板表皮开始表达,逐渐增强,从毛芽至形成毛囊维持高水平,间充质变化幅度较小;Shh则在睫毛形态发生中变化剧烈,刺激基板—毛芽—毛钉—毛囊。Shh与β-catenin睫毛形态发生中既有协同作用,也有各自的分工,Shh负责发出信号指导细胞分化决定细胞命运,β-catenin则主管维持细胞分化状态并大量增殖。提示两种信号的表达强度与精细分工差异可能是决定毛囊类型的关键因素。更多还原

【Abstract】 Ed45～240 Yak embryos eyelashes and dorsal skin were used as test and control material.Eyelash follicle morphogenesis was investigated by H.E stain and immunohistochemistry of tissue section for detecting temporal-spatial expression during morphogenesis and their reciprocal interaction rule.The epidermis dominant the changes.Mesenchymal is subordination.β-catenin expression begin from placode epidermis stage and go up increasingly as well as peak value maintain to hair follicle.The range of mesenchymal change is less.SHH expression transform is dramatically to stimulate the placode-germ-peg-hair follicle.SHH and β-catenin act cooperate interaction and special function each other.SHH is responsible for directing differentiation and determining cell fate,β-catenin charge in proliferation during cell differentiation.Our results imply SHH and β-catenin expression and detail function difference may play a key role in regulating hair follicle types.更多还原