【摘要】 目的:制备包裹基因工程MAGE3/HSP70(简称M3H)融合蛋白的长循环纳米脂质体,研究其生物学特性及抗肿瘤免疫效应。方法:采用薄膜分散-超声法,制备包裹M3H的长循环纳米脂质体(简称NL M3H),观察其形态并测量其粒径,凝胶层析法检测纳米乳剂包裹率、载药量、稳定性及体外释药特性。用NL M3H免疫小鼠,运用酶联免疫斑点法(ELISPOT)和细胞毒性杀伤实验(LDH)检测了NL M3H激活机体细胞免疫反应的状况。结果:成功地制备出平均粒径<100 nm的长循环纳米脂质体,包裹率为38%,载药量0.038 g/L,4℃放置6月后性质稳定。体外释药特性显示有74%的蛋白释放在24 h内完成。ELISPOT和细胞毒性杀伤实验显示NL M3H可以激活机体免疫反应产生针对MAGE3的CTL,特异性杀伤表达MAGE3的肿瘤细胞。结论:NL M3H具有良好的包裹率和载药量,性质稳定且具有缓释作用,可以有效激活机体免疫反应产生针对特异性抗原MAGE3的CTL,是一种很有希望的新型抗肿瘤疫苗。更多还原

【Abstract】 AIM: To prepare Nano-Liposome encapsulated MAGE3/HSP70(NL M3H) and study its character and antitumor immunity in mouse.METHODS: NL M3H was prepared by the thin film-dispersion ultrasonic.The shape and size of NL M3H were detected by electron microscope.The encapsulation rate,drug-carrying capacity,stability and the releasing character were tested by Sephedex-G100 gel filtration.The mouse was immunized by NL M3H,and the antitumor immunity was detected by ELISPOT and LDH release assay.RESULTS: The mean size of NL M3H was lower than 100 nm.The encapsulation rate was 38%.The drug content was 0.038 g/L.NL M3H has good stability after stored in 4℃ for 6 months.The releasing profile showed that 74 percent of proteins was released during the first 24 hours in saline.The results of ELISPOT and LDH release assay showed that NL M3H generated tumor specific cytotoxic T lymphocyte(CTL)to damage tumor cel1.CONCLUSION: NL M3H has novel characters,it can generate specific CTL to kill tumor cell,and can be used as new kind of vaccine agsinst tumor.更多还原