【摘要】 目的:研究SARS-CoV N蛋白与MAP19之间的相互作用。方法:利用免疫共沉淀试验验证SARS-CoV N蛋白与MAP19的相互作用,并利用Western blot检测SARS-CoV N蛋白对MAP19表达量的影响。结果:SARS-CoV N蛋白与MAP19能够在细胞内相互作用,且SARS-CoV N能够显著提高MAP19的表达量。结论:SARS-CoV N蛋白与MAP19能够在细胞内形成复合物,将为进一步研究SARS-CoV N蛋白与MAP19相互作用的生物学功能提供实验基础。更多还原

【Abstract】 AIM:Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS,an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N)protein of SARS-CoV as a bait,the N protein was found to interact with MAP19,a non-enzymatic protein of MASP(mannan-associated serine protease). The interaction between SARS-CoV N and MAP19 would be further tested in cells in this article. METHODS:The interaction between SARS-CoV N and MAP19 was demonstrated by immuno-coprecipitation,and the amount of MAP19 influenced by SARS-CoV N was investgated by Western blot. RESULTS:The interaction between SARS-CoV N and MAP19 was already demonstrated by immuno-coprecipitation. SARS-CoV N greatly increased the amount of MAP19. CONCLUSION:SARS-CoV N can bind with MAP19 in cells. Our study may be conductive to further research into the molecular mechanism of action between SARS-CoV N and MAP19.更多还原