【摘要】 采用预测食品微生物学的方法研究了副溶血性弧菌的生长规律,测定了4~40℃副溶血性弧菌的生长曲线,并在此基础上建立了副溶血性弧菌的初级和二级预测模型。结果表明,在10~40℃,副溶血性弧菌的生长曲线呈典型的S形,适合用Gompertz和logistic模型拟合;当温度在4~10℃时,适合用线性回归方程拟合。副溶血弧菌的温度模型符合Ratkowsky3式,根据预测模型,副溶血弧菌的最低生长温度Tmin为5.5℃,最高生长温度Tmax为47.3℃,最适生长温度为34.6℃。最后,对模型进行了验证。更多还原

【Abstract】 Predictive food microbiology is considered to be an effective tool for preventing the contamination of aquatic product from pathogenic microorganism.In this study,the growth characteristics and predictive model of Vibrio parahaemolyticus(V.p for short) at different temperature was studied by using the method of predictive microbiology.Growth curves were obtained at 4～40 ℃.and foud that the Gompertz model fit at 10～40 ℃ with representation of experimental curves which are typical sigmoidal.The Monod function was below 10 ℃.The variations of growth rate with temperature were modeled.and predicted that: the minimum,maximum and the optimal growth temperature is 5.5 ℃,47.3 ℃ and 34.6 ℃,respectively.The validity of the predictive model was further evaluated under controlled laboratory conditions.更多还原