【摘要】 采用料液质量比、微波功率、微波时间、水浴浸提温度、水浴浸提时间的单因素试验和正交试验来优化微波提取玉米须中水溶性多糖(CSPS)的条件。试验表明,前4种因素对多糖提取率的影响较大。微波辅助提取玉米须水溶性多糖的最佳工艺参数为:料液质量比为1 g∶50 g,在微波功率为560 W(最大700 W)的条件下提取3 min,再经100℃水浴浸提30 min。与未经微波辐照的提取方法相比较,微波辅助提取的方法时间短、产率高(最高可达2.43倍),是玉米须水溶性多糖提取的一种优选方法。然后,玉米须多糖用硫酸水解得到单糖,衍生后经GC检测,初步鉴定玉米须多糖是由葡萄糖、木糖及鼠李糖3种单糖组成。更多还原

【Abstract】 The condition of polysaccharides extraction from corn silk including solvent-to-sample ration,microwave power and time,temperature and time of water bath were studied by single factor experiment and orthogonal design.The results indicated that the former 4 factors would significantly affect the polysaccharides extraction rate.The optimum extraction conditions were as follow: solvent-to-sample of 50:1,80% microwave power(700W at full) for 3 min,and then water bath at 100℃ for 30 min.The extraction rate was bigger and the period was shorter than the process without microwave-assisted extraction technology.Finally,the corn silk soluble polysaccharides(CSPS) was preliminarily analyzed to be composed by rhamnose,arabinose and mannose,three monosaccharide,through the method of the gas chromatographic(GC) analysis.更多还原