【摘要】 目的探讨卵巢切除合并慢性铝中毒及雌激素干预对某些元素在中枢外组织分布和尿液排泄的影响。方法6月龄雌性SD大鼠40只,随机等分为卵巢假切组(Sham组)、卵巢切除组(OVX组)、卵巢切除合并慢性铝中毒组(OVX+Al组)、卵巢切除合并慢性铝中毒及尼尔雌醇灌胃组(OVX+Al+E2组)。Sham组,切除腹腔卵巢体积大小的脂肪组织,以普通饲料喂养;OVX组,切除双侧卵巢,以普通饲料喂养;OVX+Al组,双侧卵巢切除,并以25mg/g的AlCl3.6H2O的饲料喂养;OVX+Al+E2组,双侧卵巢切除,以25mg/g的AlCl3.6H2O的饲料喂养并以尼尔雌醇灌胃(1次/周,每次0.125mg/kg)。手术3个月后,代谢笼收集24h尿液,处死动物取组织。用等离子体发射光谱仪测定肝脏、肾脏、心脏、脊髓、胫骨、骨骼肌和尿的硝解溶液中某些元素含量。结果(1)与Sham组比较,OVX组的心脏Zn降低(P<0.001)。(2)与Sham组比较,OVX+Al组的心脏Zn降低(P<0.001),Si升高(P<0.05);胫骨Mg降低(P<0.05);骨骼肌Cu升高(P<0.001),Se、Si降低(P<0.001,P<0.05);脊髓Se降低(P<0.05);肝脏Cu、Zn、Fe降低(P<0.05,P<0.001,P<0.01),Ca升高(P<0.01);尿液Al、Ca、Mg升高(P<0.05,P<0.01,P<0.05)。(3)与OVX组比较,OVX+Al组的心脏Si升高(P<0.05)、Zn降低(P<0.001);胫骨Cu、Mg降低(P<0.05,P<0.05)。(4)与Sham组比较,OVX+Al+E2组的心脏Al、Cd、Si、Se升高(P<0.05,P<0.01,P<0.01,P<0.001);胫骨Se、Cd升高(P<0.01,P<0.001);肾脏Al升高(P<0.05);骨骼肌Mn、Cu升高(P<0.05,P<0.001),Se降低(P<0.001);脊髓Al、Se、Ca降低(P<0.05,P<0.01,P<0.05);肝脏Mn、Zn、Si升高(P<0.01,P<0.05,P<0.05);尿液Cd、Mg、Se、Al、Ca升高(P<0.05,P<0.05,P<0.01,P<0.001,P<0.001)。(5)与OVX+Al组比较,OVX+Al+E2组的心脏Cd、Mn、Se升高(P<0.05,P<0.05,P<0.01);胫骨Al、Mg、Se、Cd、Mn升高(P<0.05,P<0.01,P<0.001,P<0.001,P<0.001);骨骼肌Mn、Cu升高(P<0.05,P<0.001),Se降低(P<0.001);脊髓Se降低(P<0.05);肝脏Al、Ca降低(P<0.05,P<0.01),Cu、Si、Fe、Mg、Mn、Zn升高(P<0.05,P<0.05,P<0.01,P<0.01,P<0.001,P<0.001);尿液Se、Al、Cd、Mg、Si、Ca升高(P<0.05,P<0.05,P<0.05,P<0.05,P<0.05,P<0.01)。结论长期去卵巢可使大鼠心脏Zn向其它组织器官转移。长期去卵巢合并慢性铝中毒影响的中枢外主要脏器是心脏、骨骼肌和肝脏,影响的元素主要是Zn、Si、Cu和Se。慢性铝中毒可使长期去卵巢大鼠的Si向心脏转移,并进一步促进雌激素缺乏导致的心脏Zn的外转移。补充雌激素可以促进铝的尿排泄。更多还原

【Abstract】 Objective To reveal the influence of ovariectomy and chronic Aluminum toxication in combination on the distribution in tissues except nerve centre and excretion in urine of somel elements. The study will supply the basis for study of Alzheimers disease. Methods Forty 6-month-old female S-D rats were divided randomly into 4 groups:group OVX,group OVX+Al and group OVX+Al+E2. After 3 months,urine of 24h were collected by metabolic cages; Liver,kidney,heart,bone,tibia and skeletal muscle,were got from the rats. The content of the elements in these tissues and urine was detected by ICP-AES. Results Compared of group OVX with group sham,the content of Zn decreased (P<0.001). Compared of group OVX+Al+E2 with group sham,the content of Al,Cd,Si and Se in heart increased (P<0.05,P<0.01,P<0.01,P<0.001).The content of Se and Cd in tibia increased (P<0.01,P<0.001).The content of Al in kidney increased (P<0.05).The content of Mn and Cu increased (P<0.05,P<0.001),Se decreased (P<0.001) in skeletal muscle.The content of Al,Se and Ca in spinal cord decreased (P<0.05,P<0.01,P<0.05).The content of Mn,Zn and Si in liver increased (P<0.01,P<0.05,P<0.05).The content of Cd,Mg,Se,Al and Ca in urine increased (P<0.05,P<0.05,P<0.01,P<0.001,P<0.001).Compared of group OVX+Al+E2 with group OVX+Al,the content of Cd,Mn and Se in heart increased(P<0.05,P<0.05,P<0.01).The content of Al,Mg,Se,Cd and Mn in tibia increased(P<0.05,P<0.01,P<0.001,P<0.001,P<0.001).The content of Mn and Cu increased(P<0.05,P<0.001),Se decreased(P<0.001),in skeletal muscle. The content of Se in spinal cord decreased(P<0.05).The content of Al and Ca decreased(P<0.05,P<0.01),the content of Cu,Si,Fe,Mg,Mn and Zn incraesed(P<0.05,P<0.05,P<0.01,P<0.01,P<0.001,P<0.001),in liver. The content of Se,Al,Cd,Mg,Si and Ca in urine increased (P<0.05,P<0.05,P<0.05,P<0.05,P<0.05,P<0.01).Conclusion Zn in heart can transfer to other tissues after a long time ovariectomy. Heart,skeletal muscle and liver,are mainly affected tissues except nerve centre by ovariectomy and chronic aluminum toxication in combination; and mainly affected elements are Zn,Si,Cu and Se.Chronic aluminum toxication make Si transfer to heart of ovariectomized rats,and facilitate Zn in heart transfer to other tissues. Supply estrogen can promote aluminum excretion in urine.更多还原