【摘要】 为了解中国大陆地区新生隐球菌临床株基因型分布情况,我们对来自我国中东部地区的18个省、直辖市的120株血清A型和9株血清B型新生隐球菌临床株采用PCR指纹分析、IGS序列测定和MLST(Multilocus sequencing test)方法进行分析。结果显示血清A型菌株的M13指纹图谱中主要条带与VNI型标准株相同,但次要条带与VNI现有各亚型均不相符,我们将这种新VNI亚型命名为VNIc型,但VNIc型并不只分布在中国大陆,MLST分析显示中国大陆地区的VNIc型菌株同来自美国、日本、马拉维和巴西的7株菌株亲缘关系高达75,提示VNIc型可能是一种世界范围内分布的基因型;对血清A型菌株采用(GACA)4和URA-5RFLP指纹分析则呈现与VNI标准株完全一致的主次条带;9株血清B型菌株基因型为VGI型。本研究初步揭示了我国大陆地区新生隐球菌临床株的基因型分布。更多还原

【Abstract】 We analyzed the genotype of 120 Cryptococcus neoformans and 9 Cryptococcus gattii strains isolated from cryptococcosis patients residing in 16 provinces of China’s mainland,using methods of DNA fingerprint,IGS sequence,MLST and construction of dendrogram based on M13 fingerprint. 120 serotype A strains exhibited an identical M13-based VNI subtype,which was distinguishable from the reference VNI molecular type,MLST also showed the same result,for convenience we named this unique genotype VNIc.(GACA) 4 and URA-RFLP could not separate VNIc from other subtype of VNI. The 9 serotype B strains of C. gattii portrayed a typical VGI molecular type. This research showed that VNIc is responsible for the majority of clinical cryptococcosis in China.更多还原