【摘要】 分泌蛋白是指那些分泌到细胞外的蛋白质。布鲁氏菌的分泌蛋白可能介导了病原与宿主之间的相互作用,在布鲁氏菌的毒力方面发挥一定的作用,但是研究方法的局限性限制了分泌蛋白的研究。本文报道了利用蛋白质组的方法来寻找羊布鲁氏菌的分泌蛋白。首先用TCA-丙酮法提取布鲁氏菌培养上清中的分泌蛋白,双向电泳进行分离,然后用质谱来鉴定这些蛋白,最终鉴定到40种蛋白。通过生物信息学分析,发现这些蛋白主要是ABC转运系统的底物结合蛋白、外膜蛋白和热休克蛋白。这些蛋白的识别不仅有助于对布鲁氏菌致病机制的理解,而且也可为布鲁氏菌病的疫苗研制提供靶标蛋白。更多还原

【Abstract】 Secreted proteins (the secretome) of the Brucella may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. In the present study, we describe an approach to identify Brucella melitensis secreted proteins by using proteome analysis. TCA-acetone was used to recovery extracellular proteins from the culture of Brucella melitensis, and two-dimensional gel electrophoresis followed by mass spectrometry for protein resolution and identification. At last, 40 proteins were identified. These proteins included substrate-binding proteins of ABC transporters, outermembrane proteins and heat shock proteins. The identification of these secreted proteins provides important clues for the understanding of the molecular mechanism of Brucella and interesting candidates for the vaccine to treat or prevent Brucella infections.更多还原