【摘要】 利用单因子实验和正交实验对假丝酵母(Candida sp.)突变菌株YQ5摇瓶发酵产生S-腺苷甲硫氨酸的培养基成分进行了优化。单因子实验结果表明,发酵最适pH值为6.0,最佳碳源为8%蔗糖,最佳氮源为1.5%胰蛋白胨,酵母粉最适浓度为2%,MgSO4·7H2O、CaCl2、FeSO4·7H2O、CoCl2、CuSO4·5H2O、H3BO3等作为无机离子对胞内S-腺苷甲硫氨酸的积累均有促进作用。利用正交实验获得了最终的发酵培养基配方。在最适条件下发酵48h,S-腺苷甲硫氨酸含量可达1740.0mg/L。更多还原

【Abstract】 The optimized cultural medium of fermentation for Candida sp.mutant strain YQ5 to produce S-adenosylmethionine was studied.The results of single factor experiment showed that the most favorable pH value,carbon source,nitrogen source organic nutrient is 6.0,8% sucrose,1.5% tryptone and 2% yeast extract,respectively.As to inorganic salts,MgSO4·7H2O,CaCl2,FeSO4·7H2O,CoCl2,CuSO4·5H2O,H3BO3 could improve accumulation of the intercellular SAM.The ingredients of the culture medium are also opti-mized by the orthogonal experiment.Fermentation for 48 h under the optimal condition resulted in AdoMet production at 1740.0 mg/L.更多还原