【摘要】 目的探讨BCL-2和P53在地塞米松诱导胎鼠腭裂形成中的表达及意义。方法以腹腔注射醋酸地塞米松诱导胎鼠腭裂畸形,应用免疫组织化学法和图像分析研究BCL-2和P53在不同时期实验组与对照组中腭突间充质(EPM)细胞的表达情况。结果1.对照组中BCL-2表达随着腭部发育而逐渐降低;P53表达在妊娠(gestationday,GD)13.5～15.5d中随时间变化降低,而在GD16.5增高;2.实验组中BCL-2表达GD13.5～15.5降低,GD16.5中增高;P53表达GD14.5中最高,GD15.5中最低;3.与对照组比较,实验组GD14.5～15.5中BCL-2表达明显降低(P<0.05),而P53表达明显增高(P<0.01);GD16.5中BCL-2表达明显高于对照组(P<0.01),而P53表达明显低于对照组(P<0.05)。结论地塞米松诱导的腭裂与EPM细胞凋亡水平升高有关。更多还原

【Abstract】 Objective To investigate significance and effects of BCL-2 and P53 on development of cleft palate induced by dexamethasone.Methods The cleft palate of mice were got by injecting intraperitioneally with dexamethasone acetate.The different times expressions of BCL-2 and P53 in embryonic palatal mesenchyme(EPM)cell,which in the experimental groups and control group were detected by immunohistochemical method and image analysis.Results 1.In the control group,the expressions of BCL-2 gradually decreased along with the development,however the expressions of P53 gradually decreased from GD13.5 to 15.5,then increased on GD16.5.2.In the experimental groups the expressions of BCL-2 decreased from GD13.5 to 15.5,then increased on GD16.5;the expressions of P53 were the highest on GD14.5 and the lowest on GD15.5.3.Compared with control group,the expression of BCL-2 significantly decreased(P<0.05);While the expressions of P53 increased significantly(P<0.01),which happened in the experimental groups from GD13.5 to 15.5;On GD16.5 the expression of BCL-2 remained significantly higher than control group(P<0.01),however the expressions of P53 remained significantly lower than control group(P<0.05).Conclusion There is a relationship between increasing level of apoptosis and development of cleft palates induced by dexamethasone.更多还原