【摘要】 目的:研究吉西他滨白蛋白纳米粒(GEM-NP)对胰腺癌微血管生成的影响。方法:以人胰腺癌裸鼠移植瘤为对象,分为5个给药组:110 nm-GEM-NP组(n=6)、406 nm-GEM-NP组(n=6)、GEM组(n=6)、NP组(n=6)及无药对照组(n=6);运用CD34免疫组化检测微血管密度(MVD),RT-PCR法检测血管内皮生长因子(VEGF)mRNA的表达。结果:5个组给药后MVD依次为1.80±0.90、2.58±1.10、3.92±1.13、6.61±1.65及9.56±2.99;其中3个GEM阳性给药组与2个无GEM组间均有显著差异(P<0.05),110 nm-GEM-NP组与GEM组间也具有显著差异(P<0.05)。而各组间VEGF mRNA表达差异无统计学意义(P>0.05)。结论:GEM-NP对胰腺癌微血管生成有较显著的抑制效应。更多还原

【Abstract】 Objective To investigate the effect of gemcitabine-loaded albumin nanoparticles(GEM-NP) on the micro-angiogenesis of pancreatic cancer.Methods Nude mice bearing transplanted tumor of human pancreatic cancer(PANC-1 xenografts) were divided into 5 groups: 110nm-GEM-NP group(n=6),406nm-GEM-NP group(n=6),GEM group(n=6),NP group(n=6) and control group(n=6),which were separately treated with GEM-NP of 110 nm diameter,GEM-NP of 406 nm diameter,pure GEM,albumin-free nanoparticles and normal saline.The microvessel density(MVD) of the tumors in each group were assessed by immunohistochemistry method with CD34 antibody,and the expression of VEGF mRNA were detected by RT-PCR.Results MVD of the 5 groups were 1.80±0.90,2.58±1.10,3.92±1.13,6.61±1.65 and 9.56±2.99 respectively,with significant difference between the groups treated with GEM and the groups without GEM(P< 0.05),and there was also a significant difference of MVD between the 110nm-GEM-NP group and the GEM group(P< 0.05).However,there was no significant difference of the VEGF mRNA expression among these groups(P<0.05).Conclusions GEM-NP have stronger depressive effect on the micro-angiogenesis of pancreatic cancer.更多还原