【摘要】 目的:观察阻断多药耐药基因1(MDR1)表达后胰腺癌细胞对化疗药物敏感性的变化,探索提高胰腺癌化疗疗效的新方法。方法:采用RNA干扰技术,构建MDR1 siRNA重组质粒,脂质体介导转染人胰腺癌细胞株BxPC-3,并筛选稳定转染细胞克隆,RT-PCR和Western印迹法检测MDR1 mRNA和蛋白的表达变化。将细胞接种于裸鼠,观察移植瘤对化疗药物吉西他滨敏感性的变化。结果:转染MDR1 siRNA质粒后胰腺癌细胞株BxPC-3MDR1 mRNA与蛋白表达水平明显降低。体内实验结果显示,将转染前后的细胞接种于裸鼠,转染组移植瘤对化疗药物吉西他滨更为敏感(P<0.05)。结论:通过RNA干扰技术,可在转录和翻译水平降低MDR1在胰腺癌细胞株BxPC-3中的表达,并能提高细胞对化疗药物的敏感性。更多还原

【Abstract】 Objectives To observe the effect of MDR1siRNA on drug sensitivity in pancreatic cancer cell line BxPC-3, and explore the new approach of improving chemotherapeutic effect in pancreatic cancer.Methods MDR1 siRNA recombinant plasmid was constructed by RNA interference technique, and then transfected into the pancreatic cancer cell BxPC-3 by liposome.Stable transfection cell clone was screened.RT-PCR and Western blot were used to detect the MDR1 mRNA and protein expression after RNA interference.Three kinds of cells(BxPC-3, BxPC-3/vector, BxPC-3/ MDR1siRNA) were injected subcutaneously to nude mice, which were observed for drug sensitivity to Gemcitabine.Results RT-PCR and Western blot showed that the MDR1 mRNA and protein expression in the BxPC-3/MDR1siRNA group was significantly lower than the BxPC-3 and BxPC-3/vector groups.In vivo, the transplanted tumor of BxPC-3/MDR1siRNA group was more sensitive to Gemcitabine(P<0.05).Conclusions The MDR1siRNA recombinant plasmid can decrease MDR1 expression and improve chemotherapeutics sensitivity in human pancreatic cancer cell line BxPC-3.更多还原