【摘要】 背景:细胞毒性T淋巴细胞相关抗原4(CTLA-4)是一种免疫调节分子,可通过降低T细胞活性,抑制机体的抗肿瘤免疫反应。目的:探讨CTLA-4基因启动子区-1661和-318位点多态性与胃癌的关系。方法:121例无血缘关系的胃癌患者和236名正常对照者纳入研究,分别采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和扩增不应突变系统(ARMS)-PCR检测CTLA-4基因启动子区-1661和-318位点多态性。结果:胃癌组-1661位点AA基因型和A等位基因频率显著低于正常对照组(73.6%对83.9%,P=0.024;85.1%对91.1%,P=0.022);而-318位点CC基因型和C等位基因频率与正常对照组相比无明显差异。管状腺癌患者CTLA-4基因启动子区-1661位点AA基因型和-318位点CC基因型频率显著低于正常对照组(64.1%对83.9%,P=0.001;76.6%对87.3%,P=0.047)。结论:CTLA-4基因启动子区-1661位点基因多态性与胃癌呈显著负相关,-1661和-318位点多态性与管状腺癌呈显著负相关。更多还原

【Abstract】 Cytotoxic T-lymphocyte-associated antigen-4(CTLA-4) is a potent immunoregulatory molecule that suppresses antitumor immunoreaction by down-regulating T-cell activation.Aims:To investigate the association between CTLA-4 gene promoter region polymorphisms at positions -1661 and -318 and gastric cancer.Methods:Unrelated 121 gastric cancer patients and 236 healthy controls were enrolled in this study.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and amplification refractory mutation system(ARMS)-PCR were used to assess CTLA-4 gene promoter region polymorphisms at positions -1661 and -318,respectively.Results:The frequencies of AA genotype and A allele at position -1661 were statistically lower in gastric cancer patients than in healthy controls(73.6%vs.83.9%, P=0.024;85.1%vs.91.1%,P=0.022).The frequencies of CC genotype and C allele at position -318 were not significantly different between gastric cancer patients and healthy controls.Both the frequencies of AA genotype at position -1661 and CC genotype at position -318 were statistically lower in tubular adenocarcinoma patients than in healthy controls(64.1% vs.83.9%,P=0.001;76.6%vs.87.3%,P=0.047).Conclusions:CTLA-4 gene promoter region polymorphism at position -1661 shows a significantly negative association with gastric cancer,while those at positions -1661 and -318 show a significantly negative association with tubular adenocarcinoma.更多还原