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Repetitive DNA Sequences from the X Chromosome of the Spiny Eel (Mastacembelus aculeatus)

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【Author】 ZHAO Gang1,2, YU Qixing2, CHEN Ji2, WANG Boping2, ZANG Yating2, LIU Jiangdong2 1. School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430061, Hubei, China; 2. College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China

【摘要】 To investigate the characters of repetitive DNA se-quence in the sex chromosomes of the spiny eel (Mastacembelus aculeatus), the X chromosomal library was screened and a family of repetitive sequence, consisting of Ma 1-Ma 6, was isolated. The fluorescence in situ hybridization (FISH) result confirmed that Ma 1- Ma 5 dispersed over sex chromosomes and all autosomes, whereas, Ma 6 is sex chromosome-specific and distributed only on the C-band positive regions of X chromosome, and Ma 6 maybe the main components of the heterochromatic regions of X chro-mosome. This study provides additional information about the evolution of sex chromosomes in lower vertebrates such as fish.

【Abstract】 To investigate the characters of repetitive DNA se-quence in the sex chromosomes of the spiny eel (Mastacembelus aculeatus), the X chromosomal library was screened and a family of repetitive sequence, consisting of Ma 1-Ma 6, was isolated. The fluorescence in situ hybridization (FISH) result confirmed that Ma 1- Ma 5 dispersed over sex chromosomes and all autosomes, whereas, Ma 6 is sex chromosome-specific and distributed only on the C-band positive regions of X chromosome, and Ma 6 maybe the main components of the heterochromatic regions of X chro-mosome. This study provides additional information about the evolution of sex chromosomes in lower vertebrates such as fish.

【基金】 Supported by the National Natural Science Foundation of China(30400044)
  • 【文献出处】 Wuhan University Journal of Natural Sciences ,武汉大学自然科学学报(英文版) , 编辑部邮箱 ,2009年02期
  • 【分类号】Q953
  • 【下载频次】33
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