【摘要】 以2-巯基乙醇作为抗体的还原剂,葡萄糖酸钠作为中间弱配体,在pH5.0的条件下,以SnCl2快速还原188ReO4-,进行了单克隆抗体IgG的直接标记。探讨了影响标记率的因素。结果显示,优化后的标记条件为pH5.0,室温下标记3 h,SnCl2用量2.66μmol,葡萄糖酸钠用量80μmol。该方法操作步骤简单方便,标记率较高,为94.4%±0.7%,标记物不需分离纯化,且有良好的体外稳定性,室温下可稳定保存8 h。更多还原

【Abstract】 A method of direct labeling IgG with 188Re was studied.The labeling was conducted at pH5.0 with 2-ME and SnCl2 used as the reductant of antibodies and 188ReO4-respectively,and Sodium gluconate as chelate agent.The effecting factors on the labeling yield were evaluated.The labeling procedure was easy and convenient to perform,and could obtain high labeling yield(94.4%±0.7%) without further purification.The labeled antibodies were stable in vitro.Therefore the direct labeling approach has more practical application prospects.更多还原