【摘要】 利用BIOLOG测试方法对长江三角洲地区施污泥土壤的微生物群落功能多样性进行了初步探讨。研究结果表明,施用污泥后土壤微生物群落代谢剖面、多样性指数、动力学参数均发生了变化,说明微生物对单一碳源底物的利用能力发生了改变。微生物群落功能多样性动力学研究结果发现,供试土壤的微生物群落代谢剖面(AWCD)与培养时间之间呈非线性关系,其变化过程符合微生物种群生长动态模型(S型)。除滩潮土的杭州新鲜消化污泥和杭州风干污泥处理外,其他污泥处理均使土壤微生物群落功能多样性动力学参数K和r值降低,S值增大。土壤微生物群落功能多样性特征的变化可以较好地显示施用污泥后土壤微生物群落对碳源利用能力和模式的差异,反映施用污泥特定生境土壤微生物群落功能多样性的变化。更多还原

【Abstract】 The BIOLOG method was applied to exploration of functional diversity of soil microbial communities in sludge-amended soils sampled from the Yangtze River Delta.Results indicated-that metabolic profile,functional diversity indexes and kinetic parameters of the soil microbial communities changed following soil amendment with sewage sludge, suggesting that the changes occurred in population of the microbes capable of exploiting carbon substrates and in this capability as well.The kinetic study of the functional diversity revealed that the metabolic profile of the soil microbial communities exhibited non-linear correlation with the incubation time,showing a curse of sigmoid that fits the dynamic model of growth of the soil microbial communities.In all the treatments,except for treatments of coastal fluvo-aquic soil amended with fresh sludge and dried sludge from Hangzhou,kinetic parameters K and r of the functional diversity of the soil microbial communities decreased significantly and parameter S increased.Changes in characteristics of the functional diversity well reflected differences in C utilizing capacity and model of the soil microbial communities in the sludge-amended soils, and changes in functional diversity of the soil microbial communities in a particular eco-environment,like soil amended with sewage sludge.更多还原