【摘要】 将纤维素酶用于葡萄穗轴中原花色素和白藜芦醇的提取工艺中。即在乙醇提取前,先用纤维素酶处理葡萄穗轴粗粉。通过实验确定最佳的酶解条件为:酶解体系pH6,酶解温度30℃,酶解时间80 min,m(纤维素酶):m(葡萄穗轴粗粉)=1∶250。实验还对酶解后乙醇提取条件进行优化:40%乙醇溶液为提取剂,提取温度80℃,提取时间120 min。提取液通过溶剂萃取进行分离,用香草醛-盐酸法和紫外分光光度法分别测定其中原花色素和白藜芦醇的含量。与传统直接醇提法相比,原花色素的提取率提高了近4倍,白藜芦醇的提取率也有所提高。更多还原

【Abstract】 Cellulose was used in the technology of extracting proanthocyanidins and resveratrol from grape spike-stalk.The crude powder of grape spike-stalk was treated by means of cellulase before extraction by refluxing with ethanol.The optimal enzymatic conditions which were determined experimently were as follows:pH of extraction media 6,enzymatic temperature 30 ℃,enzymatic time 80 min,m(cellulose):m(grape spike-stalk)=1∶250.The optimal enzyme-based extraction conditions were 40% ethanol solution as solvent,extracting temperature 80℃,extracting time 120 min.Two products were separated by solvent extraction and analyzed by UV spectrometry and Vanillin-HCl method.Compared with traditional extracting technology by ethanol,extraction rate of proanthocyanidins was increased by about 4 times and extraction rate of resveratrol was also increased by using this enzyme-based extraction technology.更多还原