【摘要】 目的:研究沙利度胺(thalidomide)联合曲安奈德对人脐静脉内皮细胞(HUVEC)的药物作用。方法:Ⅰ型胶原酶消化法原代培养HUVEC,Ⅷ因子相关抗原检测法鉴定细胞。沙利度胺溶于0.5%二甲基亚砜(DMSO)中,分为3组。A组为沙利度胺组,分4个浓度梯度,分别为10、25、50和75mg/L;B组为沙利度胺+曲安奈德组,为含1mg/L曲安奈德的各浓度沙利度胺;C组为对照组。HUVEC传代植入96孔板,细胞密度为1×104/mL,过夜,加入不同浓度的药物,处理72h;在酶标仪570nm波长处读取吸光度(A)值。结果:2组药物均对HUVEC的增殖有明显的抑制作用(P<0.01),抑制作用与药物浓度呈正相关;与A组相比,B组对HUVEC抑制作用更强(P<0.05)。结论:沙利度胺及曲安奈德对血管内皮细胞增殖有明显作用,这两类药物在治疗新生血管性疾病中可发挥一定作用。更多还原

【Abstract】 Objective: To investigate the effect of thalidomide and triamcinolone acetonide (TA) on human umbilical vein endothelial cells (HUVEC). Methods: The primary culture of HUVEC with collagenase Ⅰ was identified with FⅧAg. There were 3 groups in the study including thalidomide group (10, 25, 50 and 75 mg/L), TA and thalidomide group and control group. The absorbance ratio was measured through MTT-test. Results: Both thalidomide group and TA and thalidomide group showed inhibitor effect on the growth of HUVEC significantly (P < 0.01). The inhibitory effect was dose dependent. Compared to thalidomide group, the inhibitory effect was more significant in TA and thalidomide group (P < 0.05). Conclusion: Thalidomide can inhibit the growth of HUVEC; however, the effect was more significant in TA and thalidomide group.更多还原