【摘要】 目的:探讨细胞外基质金属蛋白酶诱导因子(CD147)及基质金属蛋白酶9(MMP9)在乳腺癌BCML-TAII99模型移植瘤中的表达及其与肿瘤生长和转移的关系。方法:建立30只小鼠肺转移性乳腺癌BCML-TAII99模型。用免疫组织化学SP法对小鼠肿瘤组织标本CD147及MMP9蛋白表达进行检测,并分析其与肿瘤大小及转移之间的关系。结果:CD147及MMP9蛋白表达阳性率分别为63.33%(19/30)和53.33%(16/30),CD147、MMP9均阳性表达者13只;两者均阴性者8只,两者表达呈正相关(χ2=48.492,r=0.786,P=0.025)。CD147、MMP9在转移组与非转移组表达阳性率分别为92.31%、41.18%和76.92%、35.29%,差异有统计学意义(χ2=6.238,P=0.013和χ2=5.129,P=0.024)。CD147和MMP9蛋白在≥3g组和<3g组的阳性表达率分别为88.89%、25.00%和66.67%、33.33%,两者表达与肿瘤大小有关(χ2=8.452,P=0.009和χ2=4.652,P=0.039,r分别为0.469和0.366)。结论:CD147可能通过诱导MMP-9蛋白的产生在乳腺癌的浸润和转移过程中起重要作用。更多还原

【Abstract】 Objective:To study the expressions of CD147and MMP9 in the lung metastasis model of breast cancer in BCML-TAII99 mice and their correlation with tumor growth and metastasis thereof.Methods:The breast cancer model with lung metastasis was established in BCML-TAII99 mice.The immunohistochemical staining method(SP method) was used to analyze the expressions of CD147 and MMP9 in mice lung metastasis tissue.The relationship between the tumor growth and the metastasis was analyzed.Results:The positive rates of CD147 and MMP9 were 63.33%(19/30)and 53.33%(16/30) respectively in mouse models.There was significant difference in positive rate of expressions between the metastasis group and the non-metastasis group(χ2 = 6.238,P = 0.013;χ2 = =5.129,P = 0.024).There was a statistically significant positive correlation between the expression of CD147 and MMP9(r = 0.786,P = 0.025).Conclusion:The expression of CD147 may play a crucial role in infiltration and metastasis of breast cancer by inducing the expression of MMP9.更多还原