【摘要】 探讨骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植促进大鼠脊髓损伤(spinalcord injury,SCI)后行为功能恢复的机制并评价其疗效。方法:利用NYU Impactor-II打击器制作大鼠T10SCI模型,细胞移植预实验证实BMSCs在体内定植后,分别于损伤后3d(Ⅱ组)和1周(Ⅲ组)移植在体外稳定传10代的BMSCs,以DMEM作为阴性对照(Ⅰ组),术后用BBB评分和脚印分析进行大鼠行为功能的评价。3个月后处死动物,取出损伤处脊髓做10mm快速冰冻切片后,行HE,NF和ED1免疫组化染色,根据各组间阳性反应面积百分比进行比较。结果:BMSCs倍增周期约为2～3d,术后第4周各组间BBB评分差异开始有统计学意义(P<0.05或P<0.01),术后第6周起Ⅱ组和Ⅲ组之间脚印分析结果差异有统计学意义(P<0.05或P<0.01)。NF和ED1染色阳性反应面积百分比各组间差异均有统计学意义(P<0.05或P<0.01)。结论:BMSCs可以改善脊髓损伤后局部的微环境,促进轴突的再生和大鼠运动功能的恢复。更多还原

【Abstract】 Objective:To explore the mechanism of functional recovery after spinal cord injury (SCI) in rats with bone marrow mesenchymal stem cells (BMSCs) and the therapeutic effect. Methods:The rat’s SCI model was made by NYU Impactor-II at T10. After cell permanent planting was confirmed by preliminary experiment of cell transplantation, the BMSCs which passed 10 stable passages in vitro were grafted into the injured epicenter of spinal cord on 3rd d (group Ⅱ) and 1st w (groupⅢ) respectively after injury. DMEM was set as control (groupⅠ). The basso beattie bresnahan(BBB)score and footprint analysis were carried out to evaluate functional recovery of rat’s hind limbs after injury. Three months later, all rats were sacrificed. The injured spinal cord was taken out to make a 10 μm fast frozen section, which was followed by HE, NF and ED1 immunohistochemistry staining. The percentage of positive response area was assayed to compare between groups. Results: The doubling cycle of BMSCs was around 2~3 days. On the 4th w after injury, statistical difference of BBB score happened among three groups (P < 0.05 or P < 0.01), while on the 6th w after injury, statistical difference of footprint analysis appeared between group Ⅱ and group Ⅲ (P < 0.05 or P < 0.01). The statistical differences also existed between the three groups after comparing the percentage of positive response area of NF and ED1 immunohistochemistry staining (P < 0.05 or P < 0.01). Conclusion: The graft of BMSCs improves microenvironment of injured spinal cord and promotes axonal regeneration as well as functional recovery after SCI in rats.更多还原