【摘要】 目的探讨应用纳米技术抑制分枝结核杆菌生长的可行性。方法用复凝聚法制备I-1-P反义ODN-壳聚糖纳米粒子。在分枝结核杆菌菌株中分别加入不同量的自由ODN和壳聚糖-ODN纳米粒子,共同培养6周后观测细菌的生长情况。结果制得的纳米粒子由(35.6±0.9)%的ODN和(64.4±0.9)%的壳聚糖组成。壳聚糖-ODN纳米粒子浓度为4μmol/L时,其对分枝结核杆菌的抑制作用为(2.8±0.1)CFU/ml。而自由ODN在浓度低于10μmol/L时,不能有效抑制分枝结核杆菌的生长。结论壳聚糖-ODN纳米粒子比自由ODN对分枝结核杆菌的生长具有更强的抑制作用。更多还原

【Abstract】 Objective To investigate the feasibility of applying nanotechnology against M.tuberculosis growth.Methods Chitosan-antisense ODN nanoparticles were prepared by complex coacervation method.Varied amount of ODNs and chitosan-ODN nanoparticles were added to the cultures and the growth of the bacilli was monitored.Results The nanoparticles were composed of(35.6±0.9)% ODN and(64.4±0.9)% chitosan.Compared to the free ODN,antisense nanopartilces were more effective in inhibiting the proliferation of M.tuberculosis.Antisense nanoparticles decreased growth by(2.8±0.1)CFU/ml at concentration of 4 μmol/L.Conclusion Chitosan-ODN nanoparticles were more effective in inhibiting the growth of M.tuberculosis than free ODN.更多还原