Expression of Integrin-α₃ mRNA in Meningiomas and Its Correlation with Proliferation and Invasion

【摘要】 To investigate the expression of integrin-α3 mRNA in meningiomas and its correlation with proliferation and invasion, the expression of integrin-α3 subunit was detected by using in situ hybridization in patients with meningiomas (36 cases) and normal dura (2 cases) and arachnoid tis-sues (2 cases). SABC immunohistochemical assay was used to study the expression of Ki-67 nuclear antigen. The results showed that the expression of integrin-α3 mRNA in benign, atypical and malig-nant meningiomas was 2.52±0.362, 1.75±0.316 and 1.42±0.633, respectively. The expression levels of integrin-α3 mRNA was significantly lower in atypical or malignant meningiomas than those in be-nign meningiomas (P<0.05, P<0.01, respectively). The expression of integrin-α3 mRNA in those with invasive biological behavior was lower than that in those without invasion (1.63±0.462 vs. 2.61±0.526, P<0.01). Moreover, the expression of integrin-α3 mRNA was inversely associated with Ki-67 labeling index in all cases. It suggested that integrin-α3 subunit participated in the modulatory process of growth of meningiomas. The proliferation activity and malignant grade of meningiomas were increased with the decreased expression of integrin-α3 subunit, and the down-regulation of in-tegrin-α3 mRNA was associated with the invasive biological behaviors in meningiomas.更多还原

【Abstract】 To investigate the expression of integrin-α3 mRNA in meningiomas and its correlation with proliferation and invasion, the expression of integrin-α3 subunit was detected by using in situ hybridization in patients with meningiomas (36 cases) and normal dura (2 cases) and arachnoid tis- sues (2 cases). SABC immunohistochemical assay was used to study the expression of Ki-67 nuclear antigen. The results showed that the expression of integrin-α3 mRNA in benign, atypical and malig- nant meningiomas was 2.52±0.362, 1.75±0.316 and 1.42±0.633, respectively. The expression levels of integrin-α3 mRNA was significantly lower in atypical or malignant meningiomas than those in be- nign meningiomas (P<0.05, P<0.01, respectively). The expression of integrin-α3 mRNA in those with invasive biological behavior was lower than that in those without invasion (1.63±0.462 vs. 2.61±0.526, P<0.01). Moreover, the expression of integrin-α3 mRNA was inversely associated with Ki-67 labeling index in all cases. It suggested that integrin-α3 subunit participated in the modulatory process of growth of meningiomas. The proliferation activity and malignant grade of meningiomas were increased with the decreased expression of integrin-α3 subunit, and the down-regulation of in- tegrin-α3 mRNA was associated with the invasive biological behaviors in meningiomas.更多还原