【摘要】 目的观察虎杖方剂对四氯化碳(CCl4)致大鼠肝损伤的保护作用。方法参照文献方法制备CCl4致大鼠肝损伤模型,实验分正常对照组、药物对照组、CCl4肝损伤组、药物预防组、药物不同剂量治疗组。测定肝组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(AKP)活性及血清一氧化氮(NO)与肿瘤坏死因子(TNF-α)水平,并进行肝组织学检查。结果CCl4致大鼠肝损伤组肝组织SOD活性明显降低,MDA含量显著升高(P<0.01);血清ALT、AST、AKP活性及NO与TNF-α水平显著升高(P<0.01)。虎杖方剂本身对正常肝脏无损伤作用,而且虎杖方剂预防组与治疗组均能明显提高肝组织SOD活性,降低肝组织MDA含量;显著降低血清ALT,AST,AKP活性及NO与TNF-α水平(P<0.01)。肝组织学检查发现虎杖方剂能明显减轻CCl4引起的肝组织及肝细胞损伤。结论虎杖方剂本身对肝组织无明显损伤作用,通过抑制脂质过氧化、抑制TNF-α的表达和保护内皮细胞功能减轻CCl4引起的肝损伤。更多还原

【Abstract】 ObjectiveTo observe the protective effects of Huzhang Decoction(HZD)(Rhizoma Polygonicuspidati,Radix Sanguisorbae,Herba Saururi,Rhizoma curcumae,etc) on liver injury induced by CCl4 in rats.MethodsThe experimental model was established by administration of CCl4(50%,1 ml/kg,po) for 3 days.60 wistar rats were randomly divided into six groups:normal,HZD control,liver injury,HZD prevention,HZD I(treated with HZD 1.0 g/kg) and II(treated with HZD 2.0 g/kg).The activities of SOD,ALT,AST,AKP and the contents of MDA,NO and TNF-α in liver or serum were measured.ResultsCompared with normal group,HZD itself did not injury liver,but the damage induced by CCl4 was significant.The levels of SOD was obviously decreased(P<0.01),and the contens of ALT,AST,AKP,MDA,NO and TNF-αwere markedly increased(P<0.01).However,whether provention or treatment,the HZD significantly enhanced the level of SOD and reduced the contents of MDA,ALT,AST,AKP,NO and TNF-α(P<0.01).ConclusionHZD can protect liver against the injury caused by CCl4,its mechanism may be related to inbibiting lipid peroxidation and TNF-α expression,and protecting endothelial cell.更多还原