【摘要】 目的建立用高效液相色谱(HPLC)法测定川木通中豆甾醇的含量。方法色谱柱为Diamonsil C18柱(150 mm×4.6mm,5μm),流动相为甲醇-水(97∶3),检测波长为210 nm,流速1.0 ml.min-1,进样量5μl,柱温30℃。结果豆甾醇进样量在1.835~18.35μg范围内具有良好的线性关系,r=0.999 9,回收率为101.0%~103.6%,RSD为0.7%~2.1%(n=3)。结论该法准确性和重复性好,可用于川木通的质量控制。更多还原

【Abstract】 ObjectiveTo develop an HPLC method for the content determination of stigmasterol in Clematis armandii Franch.and Clematis montana Buch.-Ham.MethodsIn the system of HPLC,a Diamonsil C18(4.6 mm×150 mm,5 μm)column was used,with methanol-water(97∶3) as mobile phase.The flow rate was 1.0 ml·min-1.The detection wavelength was set at 210nm and the column temperature was 30℃.ResultsStigmasterol curve was linear over the range of 1.835～18.35 μg(r=0.999 9).The average recovery of the method was 101.0%～103.6%(RSD=0.7%～2.1%,n=3).ConclusionThe proposed method is convenient,economical and accurate with good reproducibility,and can be used to evaluate the quality of Clematis armandii Franch.and Clematis montana Buch.-Ham.更多还原