【摘要】 目的建立八珍益母浓缩丸的质量控制标准。方法利用薄层色谱法对八珍益母浓缩丸处方中益母草、党参、茯苓、甘草、白芍(酒炒)等中药进行定性鉴别;采用高效液相色谱法测定八珍益母浓缩丸中盐酸水苏碱的含量。色谱条件:Spherisorb SCX(150 mm×4.6 mm)色谱柱,以磷酸二氢钠溶液(pH3.5)为流动相,检测波长为194 nm,流速为1.0 ml.min-1,柱温为室温,进样量为20μl。结果益母草、党参、茯苓、甘草、白芍(酒炒)采用相应的薄层鉴别方法,薄层展开后均显示与对照药材相同的特异性斑点,分离效果好。盐酸水苏碱浓度在1.104~8.832μg.ml-1范围内与峰面积有良好的线性关系,回归方程为Y=533 369X+60 637,相关系数r=0.996 5(n=6)。平均回收率为98.38%,RSD为0.24%。结论该方法准确、可靠、专属性强,可用于八珍益母浓缩丸的质量控制。更多还原

【Abstract】 Objective To establish a quality standard of Bazhenyimu Concentrated Pill.Methods Traditional Chinese medicine in prescription such as herba Leonuri,Poria,Radix Paeoniae Alba were identified by a series of TLC methods.The content of stachydrine hydrochloride in Bazhenyimu Concentrated Pill was determined by HPLC method.Spherisorb SCX(150 mm×4.6 mm)column was used,the mobile phase was sodium dihydrogen phosphate buffer solution(pH3.5) at the flow rate of 1.0 ml·min-1,the detection wavelength was 194 nm,and the volume of injection was 20 μl.Results Five methods were established for the identification of Poria,Radix Paeoniae A1ba,Radix Glycyrrhizae,herba Leonuri and Codonopsis Pilosula with TLC.The principal spot in the chromatogram obtained with the test solution was similar in position,colour and size to the principal spot in the chromatogram obtained with the reference solution.The linear range of stachydrine hydrochloride was within the range of 1.104~8.832μg·ml-1.The regression equation was Y=533 369X+60 637,r=0.996 5(n=6).The average recovery was 98.38% and the RSD was 0.24%.Conclusion The method is accurate,reliable and specific and can be used for the quality control of Bazhenyimu Concentrated Pill.更多还原